Sections were stained with uranyl acetate
and lead citrate, and examined.
After uranyl acetate
staining, the pellet was rinsed with 1.
12 [micro]l of the dilutions were allowed to adsorb on carbon coated copper grid for 1 min followed by negative staining with aqueous uranyl acetate
5% glutaraldehyde for electron microscopy, embedded in Epon, sectioned, placed on grids, and stained with lead citrate and uranyl acetate
Thin sections are stained with 4% uranyl acetate
and Reynolds's lead citrate.
After staining with uranyl acetate
(3%) and lead citrate for 15 minutes, each grid was examined in a Zeiss EM 900 Transmission Electron Microscope (Carl Zeiss, Germany).
5 h and Ultrathin sections were transferred to 1 by 2 mm formvar-coated slot grids (EMS, Fort Washington, PA) and stained with uranyl acetate
and lead citrate.
Freshly glow-discharged, Formvar-coated, 400-mesh copper grids (SPI Supplies) were incubated for 10 min on the SV drops, washed three times for 10 min each in squid 1/2X buffer, and stained with 1% uranyl acetate
The fixed ovaries were contrasted in 2% uranyl acetate
in 10% alcohol during 6 hours and then dehydrated in a standard acetone series (70, 90, and 95%, five minutes each and then three changes of 100% 5 minutes each) and embedded in Epon-Araldite.
Since living specimens must be dehydrated, frozen, or fixated using a negative staining material such as uranyl acetate
or plastic embedding, this makes it difficult to see the ever-changing movements that characterize a living cell.
After dehydration, the sections were stained with uranyl acetate
5% uranyl acetate
(Fluka Chemie GmbH, Sigma-Aldrich, Buchs, Switzerland).