Urease

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urease

[′yu̇r·ē‚ās]
(biochemistry)
An enzyme that catalyzes the degradation of urea to ammonia and carbon dioxide; obtained from the seed of jack bean.

Urease

 

(carbamide amidohydrolase), an enzyme of the hydrolase class that catalyzes the breakdown of urea into ammonia and carbon dioxide. It is found in many bacteria and fungi and in some invertebrates, but is especially abundant in the seeds of such legumes as soybeans and jack beans. Urobacteria contain active urease. Urease was the first enzyme prepared in the crystalline state, by J. Sumner (1926). It is used for the quantitative determination of urea because of its high specificity.

References in periodicals archive ?
Validity (Accuracy) of rapid urease test (RUT), Gram staining, histopathology and ELISA IgG antibody were compared with culture in terms of sensitivity, specificity.
Identification was done based on Gram stain of the colony, positive oxidase, catalase and urease test [12].
Dual specimens increase the diagnostic accuracy and reduce the reaction duration of rapid urease test.
False-negative urease tests have been reported in association with several factors.
Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori.
Based on the same principle as the urea breath test, rapid urease tests detect the presence of bacterial urease in gastric mucosal biopsies.
Each patient's infection status had previously been determined by using a panel of six tests: (a) rapid urease test (CLO[R] test only), (b) antral histology with hematoxylin and eosin stain, (c) antral histology with silver stain, (d) ELISA for IgG antibody, (e) ELISA for IgA antibody, and (f) [[sup.
Eradication is defined as absence of H pylori in at least four (two from the fundus and two from the antruml samples taken from the gastric mucosa and a negative urease test.
pylori by using endoscopic biopsy specimens--(1) Rapid urease test (2) Grams staining (3) culture--most difficult yet gold standard with high specificity.
The purpose of this study was threefold: (1) to determine the prevalence of H pylori in family practice patients with dyspepsia that is unresponsive to standard empiric therapy; (2) to evaluate the accuracy of an office-based rapid urease test (also known as the campylobacter-like organism [CLO] test) in detecting the presence of H pylori compared with histologic evaluation; and (3) to assess the accuracy of an office-based serology test for IgG compared with reference laboratory serology.
Histological evaluation, culture, polymerase chain reaction (PCR) and rapid urease tests are typically performed on tissue obtained at endoscopy.