A repeat Brucella agglutination was strongly positive with 2-mercaptoethanol
(ME) titer > 1:1 280.
was positive (titer 1:320), and a 2-mercaptoethanol
test result for Brucella spp.
4], NaCl, and KCl) and other effectors (EDTA, 2-mercaptoethanol
, sodium thiosulfate) at different concentration was measured.
(Riedel-de Haen, Germany) was added in phosphate buffered saline in each well that destroyed the IgM immunoglobulins and the remaining titer was of IgG.
5), 1 mM 2-mercaptoethanol
, 10% glycerol, and bromophenol blue, and the cell extracts were subjected to SDS- polyacrylamide gel electrophoresis .
Synthetic glycopeptides and glycopeptides obtained from double proteolytic digestion were mixed with 1 [micro]L 2-mercaptoethanol
and dimethylamine to give a final concentration of 30% and incubated at 55 [degrees]C for 6 h.
The inhibitor used were EDTA, 2-mercaptoethanol
and inducer was copper sulphate.
, acetic acid, hydrochloric acid, phosphoric acid, casein, gelatin, tyrosine, 2-propanol, leupeptin, phenylmethylsulfonyl fluoride (PMSF), sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St.
2) containing 5 mmol*L-1 2-mercaptoethanol
, 1 mmol*L-1 ethylene diamine tetraacetic acid, and 7.
ARE is a thiol enzyme that is reactivated by 2-mercaptoethanol
and cysteine and acts on phenyl acetate to release phenol.
The supernatant was diluted in Laemmli sample buffer without bromophenol blue and 2-mercaptoethanol
, boiled for 3 min, and centrifuged at 12.
1g fresh leaf tissue in 4 M guanidium thiocyanate (Sigma, 59980) buffer mixed with 2-mercaptoethanol
(Sigma, M3148) at a ratio of 1:125 (2-mercaptoethanol
to guanidium) using a sterile mortar and pestle followed by the triplicate RT-PCR performed in a one-step reaction using superscript [TM] III RT/Platinum[R] Taq Mix System (Invitrogen Life Technologies) in a GeneAmp PCR system 9700 (Applied Biosystems, UK) as per Rwegasira .