At the same concentration, ascorbic acid and EEP-M protected erythrocytes against hemolysis induced by the oxidant 2,2'-azobis(2-aminopropane
) hydrochloride (AAPH) for up to 240 min, with hemolysis inhibition reaching 56.5 [+ or -] 12.8% and 37.7 [+ or -] 10.4% at 240 min, respectively, compared with erythrocytes treated with AAPH (Figure 1).
The mixture was preincubated for 20 min at 37[degrees]C, and then 19 mM of 2,2'-azobis(2-aminopropane
) (ABAP) was added.
Assessment of integral antiradical activity (IAA) of antioxidants carried by their ability to inhibit the oxidation of ABTS by peroxyl and alkoxyl radicals formed by thermal (37[degrees]C) decomposition of ABAP (2, 2'-azobis (2-aminopropane
) hydrochloride) .
Acrylic acid (AA), methylmethacrylate (MMA), 2-ethylhexylacrylate (2EHA) monomers, and the 2,2-azobis(2-aminopropane
) dihydrochloride (ABAP) initiator were of reagent grade from Wako Pure Chemical Industries, Japan.