Identification and quantification was based on external calibration with standard solutions of acetaldehyde, 2-pentanone, 2-butanone, ethylacetate (Fluka, Buchs SG, Switzerland); ethanol (Arcus, Oslo, Norway); 2-methyl-1-propanol, 2-methyl-1-butanol, 2-methyl-1-butanal, 3-methyl-1-butanol, 2-methyl-1-butanol, 2-methyl-1-propanol, 3-methyl-1-butanal, 3-methyl-1-butanol, 2-methyl-1-butanol, 2-methyl-1-propanal, diacetyl, 2-propanol, 1- butanol, 2-butanol, acetoin, iso-butylacetate, acetone, 2,3-pentanedione, ethylacetate, acetone, isobutyl acetate and ethyl butyrate (Merck KGaA, Darmstadt, Germany).
The alcohols identified in Obushera, in order of decreasing quantity, included: ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol and 2-methyl-1-butanol (Table 5).
The higher alcohols exceeded reported odour threshold values after 2 days of fermentation: 2-methyl-1-propanol (5.
Fusel oil usually includes n-propanol, 2-methyl-1-propanol, 2-methyl-1-butanol and 3-methyl-1-butanol in various concentrations.
1979) that a 5% concentration of Carbowax 20M gives the optimum resolution of methanol, ethanol, n-propanol, 2-methyl-1-propanol, 2-methyl-1-butanol and 3-methyl-1-butanol.