A high-resolution method for separating HDL into different size subfractions, 2-dimensional agarose/polyacrylamide gel electrophoresis (2D-PAGE
), was published by Castro et al.
The first proteome analysis of BALF was done in 1979 by Bell and Hook.[sup] They used 2D-PAGE
technology and compared the differential BALF proteome between normal volunteers and patients with pulmonary alveolar proteinosis.[sup] Then, Lenz et al .[sup], compared the BALF proteomes among IPF, sarcoidosis, asbestosis, and normal controls using 2D-PAGE
, 41.5 pg IgG were diluted to 1.0 mL with 0.1 M Tris-HCl, 1.0 mM EDTA, and 6.0 M guanidine at pH 8.3 and submitted to extensive reduction with DTT and carboxymethylation with iodoacetic acid according to Allen  followed by thorough dialysis against water.
The present study is an extension to our previous research and was designed to evaluate the ipsilateral cortex surrounding the lesion by analyzing the key proteins involved in PIT by 2-dimensional poly-acrylamide gel electrophoresis (2D-PAGE
) and by the peptide mass fingerprinting (PMF) analysis.
162 proteins distribute among pI 4-10 intervals but 21 proteins with pI >10 were identified that beyond the 2D-PAGE
CFTR-enriched endosomes were visualized by immuno-EM, affinity purified by AminoLink[R] gel, and analyzed by 2D-PAGE
. RESULTS: Immunoblotting showed that endosomal fraction was enriched with Rab5 (96.23+2.38%, n=6) and Rab11 (95.01+3.96%, n=6), markers of early and recycling endosomes respectively.
The following chapter provides an excellent discussion of quantitative proteomic techniques, covering 2D-PAGE
techniques, multiplexing, metabolic and postextraction labeling, and isolation and quantitation of phosphopeptides.
"Our functional genomic investigations will include quantitative measurements of mRNA using expressed sequence tags (ESTs) and microarrays, protein expression using 2D-PAGE
and mass spectrometry, and metabolite expression using various analytical platforms to gain a multi dimensional view of cellular functions and biology," notes Dr.
Recently, Saruyama and Shinbashi (1992) investigated the inheritance of a protein of 33 kDa in the [F.sub.1] generation of a cross between japonica and indica cultivars using 2D-PAGE
. Furthermore, a high molecular weight protein of 60 kDa, known as Wx protein that controls different amounts of the gene product as well as amylose content, has been identified (Sano, 1984; Sano et al., 1991).
We performed 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE
)  analysis, in duplicate, on albumin-depleted plasma.
Using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE
), we have previously demonstrated that ductal fluids contain large amounts of protein (6).