To challenge our coculture model, we additionally included (4) prohaptens (3-aminophenol, resorcinol and acetaminophen) that remained negative with regard to the induction of keratinocyte responses even after exposure in the presence of additional S9 mix (modified KeratinoSens (Natsch and Haupt, 2013)).
Representative results are shown in Figure 1 for the sensitizers cinnamic aldehyde, 2,4-dinitrochlorobenzene and 3-aminophenol, and results for 11 additional chemicals are depicted in Figure S2 (https://doi.org/10.14573/altex.1606171s).
Among the sensitizers, 3-aminophenol failed to reach the threshold value for CD86 (max.
Separately focusing on the impact of HaCaT keratinocytes on the modulation of EC[DELTA]10 and EC[DELTA]50 values for haptens or prohaptens revealed that the chemicals for which potency was decreased were mainly haptens, while the majority of the chemicals for which potency was increased are prohaptens (acetaminophen, 3-aminophenol, 2-methoxy-4-methylphenol, resorcinol, cinnamic alcohol) (Fig.
The monomers, 2-aminophenol, 3-aminophenol, or 4-aminophenol, were electropolymerized onto the working electrode from an HCI[O.sub.4] solution by continuous cycling the potential between the appropriate potential limits.
Strongly adhering polymeric films derived from 2-aminophenol, 3-aminophenol, or 4-aminophenol were desposited onto the surface of a graphite disk working electrode by sweeping the potential, covering next potential intervals: -0.2 V and +0.67 V (2-aminophenol), -0.4 V and +1.1 V (3-aminophenol) and 0.0 and +0.64 V (4-aminophenol) vs.
There is controversy in the literature about the polymerization mechanism of 3-aminophenol. This monomer was found to polymerize through the N[H.sub.2] group (18), (21) through the OH group (19).
Values of polymer mass, charge and electrochemical yield to 2-aminophenol, 3-aminophenol, and 4-aminophenol onto graphite surface.
In the NMR spectra of BABP and DABN, the protons in the residual moieties of 3-aminophenol have their chemical shifts at the same positions, and the resonance peaks at ~5.3 ppm are assigned to the protons in amino groups in BABP and DABN, respectively.
In NMR spectrum of 6FDA-6ODA-4DABN and 6FDA-ODA-BABP, chemical shifts of protons in the residual moieties of 2,6-difluoro-benzonitrile and 4,4'-difluorobenzophenone do not shift too far away from their monomers DABN and DABP, but the chemical shifts of the protons in the residual moieties of 3-aminophenol show great difference between the monomers and the polymers, which also indicates the formation of imide bonds in the polymer chains.