Keliher, "Spectrophotometric determination of nitrite with composite reagents containing sulphanilamide, sulphanilic acid or 4-nitroaniline
as the diazotisable aromatic amine and N-(1-Naphthyl)ethylenediamine as the coupling agent," Analyst, vol.
4-Aminobenzoic acid ethyl ester (98%) and 4-nitroaniline
(98%) were purchased from J&K chemicals, Beijing, China.
All chemicals used to prepare pyrazolone derivatives (ethyl cyanoacetate, aniline, 4-chloroaniline, 4-toluidine, 4-nitroaniline
, hydrazine hydrate, ethanol, sodium acetate trihydrate, sodium nitrite, and HCl) were used as received without any pretreatment.
Reaction (%) ([degrees]C) Weight time (g/mol) (h) 1 Diethylamine 37 190 (dec) 157 5 2 Pyrrolidine 46 116.5-119 155 5 3 Piperidine 63 156.4-158.7 169 5 4 Morpholine 22 178.4-180.5 171 7 5 4-Methylpiperazine 54 148 (dec) 184 7 6 Benzylamine 56 184.2-186.8 191 7 7 Aniline 75 185.3-187.4 177 7 8 4-Nitroaniline
68 145.5-148.7 222 16 9 4-Chloroaniline 80 161.9-163.3 211 10 10 4-Fluoroaniline 85 207.5-209.2 195 4 TABLE 2: Toxicity profiles predicted for compounds 4-6, metformin, and phenformin.
could utilize phenol or 4-nitroaniline
as the sole carbon source and remove PAHs [18-21].
A total of 256 bacterial isolates were collected through enrichment of the mineral salt medium with an aromatic amine, 4-nitroaniline
(100 umol L-1), and dyes (100 mg L-1) using 10 mL wastewater/sludge as inoculum source.
The compounds (1) was synthesized by the reaction of ferrocene with the diazonium salt of 2- methyl- 4-nitroaniline
. The 1H NMR spectrum of 1 showed that unsubstituted cp-ring of ferrocene displayed singlet for five protons at 4.14 ppm, however the substituted cp-ring of ferrocene splited into two signals and appeared in the region of 4.25- 4.42 ppm.
The specific activity has been defined as [micro]mole of 4-nitroaniline
released per minute by one milligram of the protease under the assay conditions.
Although the previous study had proved that eleven hydrazine derivatives, aromatic amines showed direct mutagenicity and toxicity toward Salmonella Typhimurium and associated mutagenic process such derivatives as - phenylhydrazine, 2-nitro-phenyl-hydrazine, 4-nitrophenylhydrazine, 2,4-dinitrophenylhydrazine, p-tolihydrazine, and 4-nitroaniline
One unit of trypsin activity corresponded to 1 [micro]mol 4-nitroaniline
liberated in 1 min/mL extracellular enzymatic extract, based on the extinction coefficient of the substrate ([epsilon]407 = 8,200/M/cm).
The solutions of the ionic liquid (10 m mol/L), [H.sub.2]S[O.sub.4] (10 m mol/L), and 4-nitroaniline
(10 m mol/L) were made in distilled water and their UV-vis spectra were carried out in the scale of 220-600 nm at room temperature.