The later then oxidatively couples with 4-aminoantipyrine
and phenol to generate quinoneimine.
N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (TOOS) and Good's buffer (PIPES) were supplied from Dojindo Laboratories, 4-aminoantipyrine
(4-AA) from Nittobo, and sodium azide from Nacalai Tesque.
In this reaction, hydrogen peroxide was coupled with 4-aminoantipyrine
and phenol by peroxides to produce quinoneimine dye with maximum absorption in 500nm.
2](VI) complexes with some 4-aminoantipyrine
Schiff base derivatives,Bull.
3,5-Dichloro-2-hydroxybenzene sulfonate coupled with 4-aminoantipyrine
and hydrogen peroxide, in the presence of peroxidase, forms a colored complex that is measured at 520 nm.
0), 100 mM 3, 5-dichloro-2-hydroxybenzenesulfonic acid, 10mM 4-aminoantipyrine
, and 150 [micro]L of enzyme extract (approx.
(Fluka) 3:4-methylene dioxy benzaldehyde and semicarbazide hydrochloride (all B.
4: 46 kDa, Streptomyces species) from Asahi Kasei, Good's buffer MOPS from Dojindo Laboratories, 4-aminoantipyrine
from Kanto Chemical, and N-ethyl-N-(3-methylphenyl)-N'-succinylethylene-diamine manufactured by Kyowa Medex.
The method is based on rapid condensation with 4-aminoantipyrine
followed by the oxidation with potassium ferricyanide under alkaline conditions.
MAA is further metabolized to 4-formylaminoantipyrine (FAA) and 4-aminoantipyrine
(AA), which is acetylated to 4-acetylaminoantipyrine (AAA).
75 mmol/L 4-aminoantipyrine
(as chromogen) in 50 mmol/L MES (pH 6.
2] then reacts with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-m-toluidine (EHSPT) and 4-aminoantipyrine
(4-AA) to form a quinone dye with an absorbance maximum at 556 nm.