ABCC1 expression has been shown in many species, and in human, ABCC1 mRNA is expressed in various tissues including placenta, liver, and kidney [12,13].
Although it has been shown that expression of ABCC1 gene is induced by estrogen and progesterone in human trophoblast cells , regulatory mechanisms of the expression of PG transporter genes, ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are not well understood.
Therefore, to understand the expression and regulation of PG transporters in porcine uterine endometrium during the estrous cycle and pregnancy, we evaluated i) expression of ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs in the uterine endometrium during the estrous cycle and pregnancy, in the conceptus of early pregnancy, and in chorioallantoic tissues during pregnancy; ii) localization of ABCC1 and ABCC9 mRNAs in the uterine endometrium; and iii) effects of steroid hormones, IL1B, and interferon gamma (IFNG) on ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNA expression in the endometrial tissues.
The full-length human ABCB1 cDNA in pBluescript KS (+) (Clone name: pMDRAl), human ABCC1 cDNA in pOTB7 (Clone name: RBbllBlO), and human ABCG2 cDNA in pOTB7 (Clone Name: IRAL039K21) were supplied by the Riken BRC DNA bank (Ibaraki, Japan).
ABCBl, ABCC1 and ABCG2 mRNA expression levels were quantified by real-time RT-PCR as described previously (Teng et al.
And in the recent study the researchers identified common variants at the beginning and end of the ABC1 and ABCC1 genes.
Patients who had the variant allele of either ABCB1 rs3842 or ABCC1 rs212090 had a significantly increased risk of developing lung cancer, with the former variant particularly associated with an increased risk of cancer in women and in individuals under age 60 years.
Three polymorphisms located elsewhere on ABCC1
were associated with MTX toxicity.
ATP-binding Export of organic cassette, sub-family anions and drugs + C (CFTR/MRP), member ATP-dependent 1 transport 0.
25 ng of HepG2 total RNA was created for all ABC transporters except ABCA6, ABCA8, ABCA9, ABCA10, ABCB11, ABCC1
, and ABCC7.
Multidrug resistance protein 1 (MRP1), which is structurally similar to P-gp, is the product of the ABCC1 gene and can export anthracenedione, anthracycline, epipodophyllotoxin, vinca alkaloids, etc.
The primers were: ABCB1, 3977 5'-GCTCGTCCCCTTGTTAG-ACAGCC-3' 3999 and 4186 5-CGTGCCATGCTCCTTGACTCTGC-3' 4164; ABCC1, 4381 5'-CCTGGAGCTGGCCCACCTGA-3' 4400 and 4551 5'-GCTGCCGTGGCCTCATCCAA-3 4532; ABCC2, 2075 5-GCAGCAGGTCAGAGTGTGGTTTC-3' 2097 and 2383 5-GTGATTCT-TCCACAAGCCCCAGG-3' 2361; GAPDH, 961 5-AACAGCGACACCCA-CTCaC-3' 980 and 1045 5'-CATACCAGGAAATGAGCTTGACAA-3' 1022.