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The rs4778889 T/C polymorphism was assessed by forward primer5'-CTCCACACTCAAAGCCTTTTGTTCCTATG-3' and reverse primer 5'-ATCCACGCTGGTTCCTTTCGT-3', followed by digestion with the restriction enzyme Ahdl (Fermentas, Germany).
No obstante, gracias al expediente matrimonial de Gregorio Salas y Juliana Alfaro [tambien conocida como Juliana Cespedes], iniciado en Heredia, el 30 de setiembre de 1837 (AHDL. Expedientes Matrimoniales, Caja de los anos 1836-1837), se pudo comprobar que Jose de la Cruz Cespedes habia sido hijo de un primo hermano del padre que se le asignaba oficialmente (ver Fig.
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