The blaNDM-1 gene was amplified with the following set of primers; blaNDM-1 F-5'-GGG CAG TCG CTT CCA ACG GT-3' and blaNDM-1 R5'-GTA GTG CTC AGT GTC GGC AT-3
The cadet, who will also graduate from Taiwan's Air Force Academy in late June, will start training with a T-34 Mentor and a AT-3
advanced jet trainer before his training with one of the nation's three on-duty jet fighters, F-16s, Mirage 2000s and IDFs, the sources said Sunday.
To amplify the constant sequence, primers of C-1F (5'-CAAATAAAGTTAGAGGTA GAATGT-3') and C-3R (5'-CCATAAGCACTAGCTAGCTG AT-3
') were used .
The coding region was then amplified with the desi[degrees]ned primer HK-1F: 5'-GG ATCCCTTGCCCTGTTTCTCCTGAT-3 'and HK-IR: 5'-CCATGGCTTCCCCATCAGACCGTA AT-3
' carrying the appropriate restriction enzyme sites and cloned into the pcDNA3.1 vector (Invitrogen, USA) between the BarrHI and Ncol sites, respectively.
(1996): COIe-F: 5'-ATA ATG ATA GGA GGR TTT GG-3' and COIe-R: 5-GCT CGT GTR TCT ACR TCC AT-3
Primers for PCR, which were synthesized by the Sangon Corporation (China), were as follows: 5'-GAAGCACTGTGGAA GAAGATGGAGG-3'; 5'-TCAGCAGGTAATGTTCGGGCA AT-3
' for FTO (191 bp); and 5'-AACGGATTTGGTCG TATTG-3'; 5'-GGAAGATGGTGATGGGATT-3' (208 bp) for GAPDH.
Sequences of oligonucleotide primers used in this study Gene Sequence Amplicon size 5'-GAC GAA CCA ACG GTC AGG AT-3
' chuA 5'-TGC CGC CAG TAC CAA AGA CA-3' 279 bp 5'-TGA AGT GTC AGG AGA CGC TG-3' yjaA 5'-ATG GAG AAT GCG TTC CTC AAC-3' 211 bp 5'-GAG TAA TGT CGG GGC ATT CA-3' TSPE4.C2 5'-CGC GCC AAC AAA GTA TTA CG-3' 152 bp Gene Reference chuA (Clermont et al., 2000) yjaA (Clermont et al., 2000) TSPE4.C2 (Clermont et al., 2000) Table 2.
Venus still shines at-3
.9 this month and grows only a little in angular diameter --from about 11W to 13", while shrinking only a little in phase--from 89% to 80% illuminated.
They left behind a stash of arms, including AT-3
Sagger, anti-tank guided missiles and ammunition.
The Stroop test, PAS AT-3
(Turkish), and BICAMS battery (symbol digit modality test, SDMT; California verbal learning test-II, CVLT-II; brief visuospatial memory test revised, BVMTR) were used to assess cognitive performance.
The V4-V5 region of the bacterial 16S ribosomal RNA gene was amplified by PCR (95[degrees]C for 30 s, 55[degrees]C for 30 s, and 72[degrees]C for 45 s and a final extension at 72[degrees]C for 10 min) using primers 338F 5,-barcode-ACTCCTACGGG AGGCAGCA-3' and 806R5'-GGACTACHVGGGTWTCTA AT-3
' , where barcode is an eight-base sequence unique to each sample.