Absorption Microscopy

The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Absorption Microscopy


a method of studying the structure and chemistry of cells in animal and plant organisms with the aid of a microscope through selective absorption by internal cell structures.

Ultraviolet rays are absorbed by the cyclic groupings of nucleus cells and other nucleotides, by proteins, some vitamins, and steroles; visible light is absorbed by pigments. By illuminating the microscope apparatus with rays in a narrow band of the spectrum in the absorption band of some given substance, the distribution of this substance in the cell and the structure of the cell can be studied. The attenuation of the beam of light that passes through the structure is a measure of the concentration of the substance absorbing the light. The possibilities of absorption microscopy are increased by the use of stains that are specifically associated with the various substances of the cell.


Metody tsitologicheskogo analiza. Moscow, 1957. (Translated from English.)
Brodskii, V. Ia. Trofika kletki. Moscow, 1966.


The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
References in periodicals archive ?
Nagai, "Spectral fingerprinting of individual cells visualized by cavity reflection enhanced light absorption microscopy," PloS One, vol.