Chemical constituents and antifilarial activity of Lantana camara against human lumpatic filariid Brugia malayi and rodent filariid Acanthocheilonema
viteae maintained in rodent hosts.
A pan filaria IgG-detecting assay (Acanthocheilonema
viteae ELISA Kit; Bordier Affinity Products SA, Crissier, Switzerland) showed a positive result.
The primers used target a 724 bp fragment of the mitochondrial cytochrome oxidase subunit I gene and are specific for various filarioid helminths (e.g., Dirofilaria, Wuchereria, Brugia, Onchocerca, Setaria, and Acanthocheilonema
and occasionally by Acanthocheilonema
Pleural effusion associated with Dipetalonema perstans (Acanthocheilonema
ES-62 was purified to homogeneity from spent culture medium of adult Acanthocheilonema
viteae using endotoxin-free reagents as described previously .
Further evidence comes from 454 pyrosequencing that identified Wolbachia genes in two naturally Wolbachia-free filarial nematodes: Acanthocheilonema
viteae and Onchocerca flexuosa (McNulty et al., 2010).