At the end of each exposure time, the lady beetles were frozen to death; the next day the legs and wings were removed (in the case of adults) to reduce the amount of residue after the insects underwent
acetolysis, which was conducted in the same place where the floral buds also underwent
acetolysis procedure, using the same method.
Acetolysis and treatment with NaOH are known to dissolve the exine of Xyridaceae pollen (Erdtman, 1952; Carlquist, 1960), thus only untreated grains were examined.
Mannan can thus be fragmented by
acetolysis, which selectively cleaves the backbone a-1,6-linked mannose units (Fig.
Asia###14.06.1997, F.Gunes (Wodehouse and
Acetolysis).###
The organic fraction was then treated by standard
acetolysis according to Moore et al.
The floral origin of honeybee-collected pollen pellets Was identified by color and light microscope examination by palynological analysis according to the
acetolysis method [9].
For pollen analysis 1 [cm.sup.3] block samples from every 10 cm were treated with a 10% KOH solution followed by standard
acetolysis according to Moore et al.
Success in identification can be improved by using
acetolysis on the pollen grains prior to mounting them on slides (Kearns and Inouye, 1993).
Of the thirty-nine sediment samples taken from Pit C, twenty were processed according to standard Erdtman
acetolysis procedures, following carbonate removal with 10% HCI.
For pollen analysis a dried sample of 50 mg was treated with 10% HCl, followed by the standard
acetolysis of Moore & Webb (1978).
Next, each sample was washed with distilled water, centrifuged and then extraneous organic material was oxidized using the
acetolysis process consisting of a mixture of sulfuric acid and acetic anhydride (Erdtman 1960).