Two Mountain Whitefish (2/59; 3.4%; 95% CI 0.9-11.5%) and 2 Rainbow Trout (2/61; 3.3%; 95% CI 0.9-11.2%) were identified as potential positives by the presence of gross internal tissue nodules and acid-fast bacteria in tissue smears (Fig.
Two Mountain Whitefish samples from the 2009-2010 collection, positive for acid-fast bacteria by tissue smear, were examined by histology at WADDL.
Effects of extracts from seaweeds on the growth of some
acid-fast bacteria," Nippon Saikingaku Zasshi, 11, pp.
blood cultures positive for 2/0
acid-fast bacteria, aerobic/anaerobic Time of collection of first 8/11 positive/last positive blood culture, d after readmission Outcome Resolved after antimicrobial drug treatment Characteristic Patient 2 Age, y/sex 2.0/F Comorbidity Down syndrome Time between surgery and 97 readmission, d Position of vegetation Septal cusp of tricuspid valve and VSD patch Hemodynamic consequence Moderate tricuspid valve regurgitation No.
Culture for common bacteria and fungi was negative, but culture of a sterile nodule aspirate on Lowenstein-Jensen medium was positive for
acid-fast bacteria after 5 weeks.
Ziehl-Neelsen staining showed an intracellular accumulation of
acid-fast bacteria in several alveolar macrophages and epithelioid cells.
Special stains for fungi and
acid-fast bacteria were negative.
Various organs and tissues from seven banded mongooses and a suricate were fixed in 10% buffered Formol-saline, embedded in paraffin wax, cut into 4-[micro]m sections, and stained with hematoxylin and eosin, as well as Kinyoun's method for acid-fast bacteria (AFIP modification) (7).
Acid-fast bacteria were detected on Ziehl-Neelsen-stained impression smears, and Mycobacterium species were isolated from both banded mongooses and the suricate specimens.
They discussed
acid-fast bacteria, nontuberculous mycobacteria, gram-positive bacteria, and fungi as possible causes (3) but more recently concluded that the specific etiologic agent(s) for HP among workers exposed to (MRF) aerosol remain(s) unknown (2).
