In pancreatic cells, STZ has the ability to act as NO donor (Som et al, 2001), which prevents mitochondrial matrix aconitase
activity, leading to DNA alkylation and damage increases the activity of guanylyl cyclase and the formation of cGMP, which are characteristic actions of NO.
(17,22) In healthy prostatic epithelial cells the presence of high levels of zinc inhibit the enzyme aconitase
, thereby preventing the oxidation of citrate in the Krebs' cycle.
Curcumin attenuates Cr(VI)-induced ascites and changes in the activity of aconitase
and F(1)F(0) ATPase and the ATP content in rat liver mitochondria.
Citrate is converted into isocitrate by the aconitase
(AC), which is further converted to [alpha]-Ketoglutarate via the isocitrate dehydrogenase (IDH) producing NADH from NAD in the process.
White, "Loss of lung mitochondrial aconitase
activity due to hyperoxia in bronchopulmonary dysplasia in primates," American Journal of Physiology Lung Cellular and Molecular Physiology, vol.
The mitochondrial enzymes involved in these reactions are (iso)citrate dehydrogenase (IDH2) and aconitase
MacLeod, "Regulation of mitochondrial aconitase
by phosphorylation in diabetic rat heart," Cellular and Molecular Life Sciences, vol.
These findings might have been indicative of altered glutaminolysis (the generation of [alpha]-KG from glutamine and GLU), which is a critical metabolic process for most tumors owing to aconitase
During this process, fumarate reacts with thiol groups of cysteine residues to produce S-(2-succino)-cysteine (36), which has the potential of affecting many proteins, including mitochondrial aconitase
and Kelch-like ECH-associated protein-1 (KEAP1) (37).
Inhibition by alloxan of mitochondrial aconitase
and other enzymes associated with the citric acid cycle.
MitoNEET (mitochondrial Asn-Glu-Glu-Thr), an iron-sulfur- (Fe-S-) containing mitochondrial outer membrane protein that is involved in transferring the Fe-S clusters to the cytosolic aconitase
, had been identified by several research groups as a binding target of the insulin sensitizer pioglitazone [37-39].
Huang, "Inhibitory effect of homocysteine on rat neural stem cell growth in vitro is associated with reduced protein levels and enzymatic activities of aconitase
and respiratory complex III," Journal of Bioenergetics and Biomembranes, vol.