SXSM-enhanced TCA cycle due to increased aconitate
hydratase, succinyl-CoA ligase (GDP-forming) subunit alpha and beta (SUCLG1/2) and succinyl-CoA ligase (ADP-forming) subunit beta (SUCLGA2), isocitrate dehydrogenase2, and dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT, PDHB).
hydratase; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Ferritin repressor protein; AltName: Full=Iron regulatory protein 1; Short=IRP1; AltName: Full=Iron-responsive element-binding protein 1; Short=IRE-BP UniProtKB/Swiss-Prot: Q01059.
5) and 100 [micro]M NaCl was used, and the reaction was initialized with 200 [micro]M aconitate
Its toxicity is caused by sodium monofluoroacetate (MFA), which inhibits aconitate
hydratase, one of the tricarboxylic acid cycle enzyme (LEE et al.
Three (30%) patients were suspected to have mitochondriopathy based on biochemical parametres which included elevated lactate in urine or plasma, elevated alanine in plasma amino acids, urine GC-MS showing elevation of one or more of the following compounds; Pyruvate, Succinate, Fumarate, Citrate and Aconitate
(a metabolite in Kreb's cycle).
Due to the lack of Krebs cycle in mitochondria, the detection of aconitase and isocitrate dehydrogenase might suggest the existence of [alpha]-ketoglutarate as a metabolite of aconitate
The root tips of ES8 and ET8 had similar organic anion profiles, with no significant differences in exudation of citrate, malate, aconitate
, succinate, formate, or acetate between the ES8 and ET8line.
radioresistens 0 90 0 0 0 Trans- Phenyl Amino- Species Malonate aconitate
* alanine * butyrate * A.
11 HCL-KCL buffer HCL-Glycine buffer HCL-Phthalate buffer Aconitate
buffer Citrate buffer Acetate buffer Citrate-phosphate buffer Succinate buffer Phthalate-NaOH buffer Maleate buffer Cacodylate buffer Phosphate buffer Tris(hydroxymethyl)aminomethane -maleate buffer Barbital buffer Tris buffer Boric acid-borax buffer Propanediol buffer Glycine buffer Borax-NaOH buffer Carbonate-bicarbonate buffer pH 1.
Enzyme System AAT Aspartate aminotransferase ACO Aconitate
hydratase ALD Fructose-bisphosphate aldolase AP-1 Glycil-leucine peptidase ARK Arginine kinase DIA Dihydrolipoamide dehydrogenase EST Esterase [beta]-GAL [beta]-galactosidase GAPDH Glyceraldehide-3-phosphate dehydrogenase GPI Glucose-6-phosphate isomerase G3PDH Glycerol-3-phosphate dehydrogenase NAD (+) IDH Isocitrate dehydrogenase NADP (+) LAP-1 Leucine aminopeptidase-1 LAP-2 Leucine aminopeptidase-2 MDH Malate dehydrogenase ME Malate dehydrogenase NADP (+) MPI Mannose-6-phosphate isomerase ODH D-octopine dehydrogenase PGD 6-phosphogluconate dehydrogenase PGM Phosphoglucomutase PP Phenyl-proline peptidase SOD Superoxide dismutase Abbr.
We analyzed seven different enzyme systems: aconitate
hydratase (Acn), diaphorase (Dia), isocitrate dehydrogenase (Idh), malate dehydrogenase (Mdh), phosphoglucoisomerase (Pgi), phosphoglucomutase, (Pgm) and Triose-phosphate isomerase (Tpi).
Basal metabolism Aconitate
hydratase Lipase Fructose-1,6-biphosphatase F-II Fructose-bisphosphate aldolase Alpha-amylase B 3-oxoacyl-ACP reductase C-4 sterol methyl oxidase 2 Amine oxidase Scavenger receptor cysteine-rich protein Vacuolar H+-ATPase C chain 4.