Based on the previous classification, Table III shows the percentage of intact sperm cells that underwent capacitation along with acrosomal reaction
in fresh samples and after incubation in presence of PVL.
However, progesterone could induce sperm chemotaxis towards oocyte and acrosomal reaction
Inoue and Lewis Tilney (1982) observed the acrosomal reaction
of the sea cucumber sperm (Fig.
Spontaneous acrosomal reaction
in the fresh sperm was not affected by EDTA or Trolox; however, EDTA decreased acrosome loss in the FT sperm.
gigas, fertilization process requires acrosomal reaction
to bind and penetrate the oocyte (Akcha et al.
Supravital staining, hypo-osmotic swelling test and normal acrosomal reaction
analysis tests were also performed for viability, plasma membrane and acrosomal integrity, respectively.
As we know, normal fertilizing capacity of sperm depends on normal acrosomal reaction
and normal functions of acrosomal enzymes.
Biomolecular research proves that with the additional of several seminal plasma proteins can improve the fertilization process, cell survival and has a positive role to the process of spermatozoa capacitation and acrosomal reaction
Only capacitated cells can undergo acrosomal reaction
, a vital step that aids the cell to penetrate the ova to achieve fertilization.
It also prevents efflux of cholesterol from the sperm membrane and MDA production in diluents indicates it prevents premature capacitation and acrosomal reaction
as act as antioxidant [30, 69].
Adegoke, "Calcium antagonists modulate oxidative stress and acrosomal reaction
in rat spermatozoa," Archives of Medical Science, vol.
Healthy spermatozoa must retain this membrane throughout freeze-thaw process and start acrosomal reaction
in female tract (Therien and Manjunath, 2003).