Streptomycin thallous acetate actidione
agar (Oxoid, UK) was used for Brochothrix thermosphacta (B.
To compare two different culture media, namely Sabouraud's Dextrose Agar (SDA, Hi-Media Laboratories, Mumbai) with Actidione
and Dermatophyte Test Medium (DTM, Hi-Media Laboratories).
Out of the 64, 20 were carbohydrate assimilation; 4 were phosphatase, urea, nitrate, and actidione
Samples suspicted of containing superficial mycosis such as skin samples were cultivated in SDA that included actidione
(0.1 g/L) in addition to the same antibiotics, and incubated at 37[degrees]C and 27[degrees]C for 21-30 days.
Populations of microorganisms were determined in triplicate by the spread plate method using the following microbiological media: organotrophic bacteria--a medium with an addition of soil extract according to Bunt and Rovira (1955); oligotrophic and copiotrophic bacteria a medium with a peptone and meat extract according to Onta and Hattori (1983); actinomycetes--Kuster and Williams medium with antibiotics (nystatine and actidione
) (Parkinson et al.
(1977) containing Chloramphenicol and Actidione
. Half of the inoculated plates were incubated at 37[degrees]C, while remaining were left at room temperature (28-32[degrees]C) for a period of 5-7 days.
[sup] The authors conclude that glucocorticoids have nongenomic effects in airway smooth muscle cells because the application of RU486, the selective glucocorticoid receptors antagonist, and actidione
, the blocker of protein synthesis, did not affect the spasmolytic effect of glucocorticoids.
A progressive increase in the total yeast count (from WLN medium), a stable number of wild yeasts resistant to actidione
(from WLD medium) and a low number of non-Saccharomyces (from Lysine Agar) demonstrated that the number of Saccharomyces increased considerably with the addition of sugar cane juice (Figure 1).
API ID32 C strips have 30 coupules containing different carbohydrates and one containing actidione
Brettanomyces bruxellensis is known to be highly resistant to cycloheximide (sold commercially as Actidione
), which can be a useful factor, but quite a few other non-Saccharomyces wild yeasts are also resistant.
Identification: Species identification of yeast isolates was done by standard procedures including morphology, germ tube test, cornmeal agar test (Hi-Media, Mumbai, India), triphenyl tetrazolium chloride reduction (Hi-Media Mumbai, India), assimilation of various sugars, and growth in presence of actidione
(50 mg/L) was added after sterilisation of the medium by autoclaving.