Plants were dissected into flowers, bracts, fleshy roots with adventitious buds
, and predominantly haustoria-enriched roots, immediately frozen and homogenized in liquid nitrogen, and stored at -80[degrees]C.
In Guzmania "Hilda", the regeneration of adventitious buds
into plantlets was obtained on culture medium supplemented with NAA plus TDZ (HUANG et al., 2011b).
(2009) detected that in this explant the adventitious buds
development occurred always at the hypocotyl end.
It is important to point out that internodal segments did also produce adequate adventitious bud
in our study.
After 4 weeks' culture, induction of organogenesis was observed and the number of adventitious buds
, which can grow in opposite sides (Fig.
Forest Product Laboratory's Wood Handbook, is a "hard, woody outgrowth on a tree, more or less rounded in form, usually resulting from the entwined growth of a cluster of adventitious buds
Buds that develop on the plant at positions other than the leaf axil are termed adventitious buds
. These buds may develop on stems, roots, or leaves, producing new shoots from any of these positions.
Tang, Ishii and Ohba (1996) observed similar results; during the initiation culture on a basal medium WPM, containing 2-8 [micro]M of BA, it was observed that epicotyl explants of Alnus cremastogyne did not elongate, and adventitious buds
and shoots were not formed.