affinity chromatography


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Related to affinity chromatography: Ion exchange chromatography

affinity chromatography

[ə′fin·əd·ē ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.
References in periodicals archive ?
Frontal analysis is a second method that is often used in affinity chromatography and HPAC to characterize biological interactions (7, 8, 10).
A and C) F2 Chromatographic profile of F2 of chia commercial seeds and flour (Salvia hispanica L.), respectively, in affinity chromatography trypsin-sepharose CNBr 4B.
Porath, "Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography," Proceedings of the National Academy of Sciences of the United States of America, vol.
Both PVA-GA-Con A and PVA-GA-PpeG proved to be promising and attractive due to the low cost affinity chromatography supports for glucose/mannose-bearing glycoconjugates and glucose/mannose-specific lectins, respectively.
Affinity Chromatography: A review of Clinical Applications.
At this point, we noticed the potential of affinity chromatography as an informational tool.
1 shows the elution profile of enzyme activity and protein content from the procainamide-based affinity chromatography. AChE activity was detected in the washed fraction and this was due to column overloading.
[16] Amersham Biosciences, Ed., Affinity Chromatography: Principles and Methods, Freiburg, Germany, AD, 2002.
Recombinant Xn-relE was purified with glutathione S-transferase (GST) tag present at the N-terminus using glutathione sepharose affinity chromatography column as per the manufacturer's instructions.
IgG from immunized rabbit serum sample at day 60, were purified by affinity chromatography using protein A column.
Summary: Zn++-dependent acid phosphatase from camel liver was partially purified 333-fold by ammonium sulfate precipitation, ion exchange chromatography on DEAE- Cellulose and SP Trisacryl columns, gel filtration on Sephadex G-100 and by affinity chromatography on Sepharose 4B-L-Tartramic amide column.