affinity chromatography


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Related to affinity chromatography: Ion exchange chromatography

affinity chromatography

[ə′fin·əd·ē ‚krō·mə′täg·rə·fē]
(analytical chemistry)
A chromatographic technique that utilizes the ability of biological molecules to bend to certain ligands specifically and reversibly; used in protein biochemistry.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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References in periodicals archive ?
Frontal analysis is a second method that is often used in affinity chromatography and HPAC to characterize biological interactions (7, 8, 10).
Analysis of Biological Interactions by Affinity Chromatography: Clinical and Pharmaceutical Applications
A and C) F2 Chromatographic profile of F2 of chia commercial seeds and flour (Salvia hispanica L.), respectively, in affinity chromatography trypsin-sepharose CNBr 4B.
Anti-tryptic activity in seed and food product of chia (Salvia hispanica L.)/Atividade antitriptica em semente e produto alimenticio de chia (Salvia hispanica L.)
Porath, "Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography," Proceedings of the National Academy of Sciences of the United States of America, vol.
Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles
Both PVA-GA-Con A and PVA-GA-PpeG proved to be promising and attractive due to the low cost affinity chromatography supports for glucose/mannose-bearing glycoconjugates and glucose/mannose-specific lectins, respectively.
PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography/PVA-Glutaraldeido como suporte para imobilizacao de lectina e cromatografia de afinidade
Purification was carried out and evaluated in an affinity chromatography system.
Clean solutions fuel affinity chromatography: when using affinity chromatography to isolate proteins from solutions, ultrapure water and contamination-free chemicals are essential for reproducible data
Affinity Chromatography: A review of Clinical Applications.
Diseno asistido por computadora de la inmovilizacion covalente de bromelina y papaina
At this point, we noticed the potential of affinity chromatography as an informational tool.
Frontal affinity chromatography: a unique research tool for biospecific interaction that promotes glycobiology
1 shows the elution profile of enzyme activity and protein content from the procainamide-based affinity chromatography. AChE activity was detected in the washed fraction and this was due to column overloading.
Assessment of Acetylcholinesterase from Channa micropeltes as a Source of Enzyme for Insecticides Detection
[16] Amersham Biosciences, Ed., Affinity Chromatography: Principles and Methods, Freiburg, Germany, AD, 2002.
Development and validation of an affinity chromatography-protein G method for IgG quantification
Recombinant Xn-relE was purified with glutathione S-transferase (GST) tag present at the N-terminus using glutathione sepharose affinity chromatography column as per the manufacturer's instructions.
Expression, purification, and functional analysis of Novel RelE Operon from X. nematophila
IgG from immunized rabbit serum sample at day 60, were purified by affinity chromatography using protein A column.
Production of polyclonal antibody against M13 phage for application in nanobody technology
Summary: Zn++-dependent acid phosphatase from camel liver was partially purified 333-fold by ammonium sulfate precipitation, ion exchange chromatography on DEAE- Cellulose and SP Trisacryl columns, gel filtration on Sephadex G-100 and by affinity chromatography on Sepharose 4B-L-Tartramic amide column.
Zinc Dependent Acid Phosphatases from Camel Liver: Purificaion and Chracterization

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