The estimate of residual variance (variability between aliquots
) obtained from model (5) was used to calculate the repeatability of nitrogen contents according to each treatment as follows:
For each repetition, 1.8L of pasteurized milk, abiding legal parameters and adulterant-free, was homogenized and divided into six 300-mL aliquots
. Different concentrations of sodium hydroxide (0.05, 0.1, 0.25, 0.5, and 1%) were separately added to five of the aliquots
, and one aliquot
was assigned as the negative control.
(8) This procedure results in an S/CO ratio that is lower for the G aliquot
than for the PBS aliquot
These have been efficiently applied to perform various syntheses and processes that were still followed by withdrawing aliquots
The supernatants were combined and frozen in aliquots
The extension requires two additional steps to the traditional transformation protocol: to allow the students time to treat their DNA sample and then to have more plates available for the students to transform the appropriate number of aliquots
of competent cells (usually six: no DNA negative control, positive control of plasma with no treatment, amylase, protease, DNase, and RNase treatment).
The latter suspension was distributed in four 100-[micro]L aliquots
: 2 aliquots
were incubated with heparin and constituted the treatment groups in chlortetracycline stain (CTC) and indirect immunofluorescence (IIF) trials; the other two were not incubated with heparin and constituted the respective control groups.
RESULTS: A total of 126 red blood cell transfusion aliquots
were used over the three month period.
Initially an aliquot
of VMS is taken, to which, suppressor is added to ensure the deposition is inhibited to the furthest possible extent (brightener intercept solution).
Homogenates were sedimented at 26,000 x g for 10 min to remove storage vesicles containing catecholamines, which interfere with TH activity, and assays were conducted with 100 [micro]L aliquots
of the supernatant solution in a total volume of 550 [micro]L Each assay contained final concentrations of 910 [micro]M FeS[O.sub.4], 55 [micro]M unlabeled L-tyrosine (Sigma), 9.1 [micro]M pyridoxal phosphate (Sigma), 36 [micro]M [beta]-mercaptoethanol, and 180 [micro]M 2-amino-6,7-dimethyl-4-hydroxy5,6,7,8-tetrahydropteridine HCl (Sigma), all in a buffer of 180 mM sodium acetate and 1.8 mM sodium phosphate (pH 6.1).
from dilutions of selected Aroclors in transformer oil (SRMs 3077 and 3075) with toluene were analyzed directly (no cleanup) by GC-ECD using conditions used for oil analyses  and a DB-5 column.
Broth cultures were further incubated at 37[degrees]C for 48 h, and 0.1 mL aliquots
were spread onto duplicate plates of VRE (commercial denomination product, Oxoid) agar.