After washing in PBS, to visualize the plasma membrane, the Golgi area and Tomes processes of the ameloblasts
at each stage of cellular differentiation (Akita et al., 1988; Kagayama et al., 1997; Chazotte, 2011), the sections were then incubated with wheat germ agglutinin (WGA) conjugated fluorescein isothiocyanate (FITC) (J-Chemical., Tokyo, Japan) diluted 10 [micro]g/mL for 10 min at room temperature.
Follistatin regulates enamel patterning in mouse incisors by asymmetrically inhibiting BMP signaling and ameloblast
(b-c) Highlight for the columnar basal cells of similar to ameloblasts
and the overlying layers loosely arranged with several amounts of ghost cells (H/E, 200x).
quantitative defect (deficient enamel ma- trix formation) due to disruption of the secre- tory phase during ameloblast
Researchers at the Forsyth Institute in Massachusetts, a fluoride research center for the past century, found that fluoride initiates an ER stress response in ameloblasts
that interferes with protein synthesis and secretion--culminating in dental fluorosis.
(9,10) The undulating course of ameloblast
during amelogenesis results in the formation of a specific pattern by so, the course of enamel rods is not the same throughout the thickness of enamel.
Cytokeratin 19, which is strongly expressed in preameloblasts and secretory ameloblasts
, is not expressed in odontomas.
This may reflect the direct effect of these antibiotics on active ameloblasts
or may be attributed to the infectious disease for which antibiotic was prescribed.8,12,23
Stress Response Pathways in Ameloblasts
: Implications for Amelogenesis and Dental Fluorosis.
Bmp2 is downregulated at the time of terminal differentiation of ameloblasts
, suggesting that the differentiation process is affected in the CCOC cells.
The enamel is produced by ameloblasts
(Figure 2D) originated from the inner epithelium of the enamel organ, which is originated from the dental lamina.