After 5 minutes incubation at room temperature, cobalt chloride solution was replaced with freshly prepared solution containing equal volumes of ammonium molybdate
solution (6.25%) and ammonium vanadate solution (0.42%).
100 g of seeds received, separately and/or combined, the application of 0.61 g peat inoculant based on Azospirillum brasilense; 0.61 g of peat inoculant based on Rhizobium tropici; and 0.07, 0.14, 0.21 and 0.28 g of ammonium molybdate
. These quantities were established based on the doses of 100 g of inoculant for 60000 seeds and 7.5, 15, 22.5 and 30 g Mo [ha.sup.-1].
A 0.68 M solution of ammonium molybdate
tetrahydrate (Sigma-Aldrich 277908) was prepared in a mixture of distilled water and dimethylformamide in a ratio of 5:1, respectively.
Reagents: 4 % ammonium molybdate
solution and 0,03 % hydrogen peroxide solution.
The color was developed with molybdate reaction which was as follows: The 500 L mixture (composed of 200 L of 2 % ammonium molybdate
and 300 L of 14 % ascorbic acid in 50 % trichloroacetic acid) was added to the above mixture (700 L) followed by the addition of 1 mL solution containing 2 % trisodium citrate and 2 % sodium arsinate in 2 % acetic acid to make the total volume of 2200 L.
The second solution of lower reactant was prepared by adding ammonium molybdate
and ammonium nitrate each weighing 817 mg to 80 mL of distilled water.
For example, for the phosphate, while shaking, five drops of reagent 9 (ammonium molybdate
solution) and five drops of reagent 10 (4-methyl-aminophenol sulfate, sodium bisulfite) were added in reaction vessel; after five-minute incubation the newly appearing colour was matched with the kit colour scale for the semiquantitative analysis.
Blank 1 contains 0.5 mL substrate (65 mM hydrogen peroxide in 60 mM sodium-potassium phosphate buffer, pH 7.4), 0.5 mL 32.4 mM ammonium molybdate
solution, and 0.1 mL kidney homogenate; Blank 2 contains 0.5 mL substrate, 0.5 mL ammonium molybdate
solution, and 0.1 mL sodium-potassium phosphate buffer; Blank 3 contains 0.6 mL sodium-potassium phosphate buffer and 0.5 mL ammonium molybdate
Plasma phosphorus is quantified through reaction with ammonium molybdate
to form a colored phosphomolybdate complex (3).
A negative stain composed of 5% ammonium molybdate
, pH 6.9, and 0.1% trehalose (wt/vol) was briefly applied to the grid and blotted.
To produce donut-like SrMo[O.sub.4], 5 mmol each of strontium salts [A = Sr[(N[O.sub.3]).sub.2], B = Sr[(C[H.sub.3]C[O.sub.2]).sub.2], and C = Sr[Cl.sub.2] x 6[H.sub.2]O] and 5 mmol ammonium molybdate
tetrahydrate [[(N[H.sub.4]).sub.6][Mo.sub.7][O.sub.24] x 4[H.sub.2]O] were separately dissolved in 25 mL distilled water to form strontium and molybdenum solutions, which were mixed, stirred for 10 min at room temperature, and processed by a 270 W microwave-hydrothermal method for 5, 15, 30, and 90 min (encoded as 1, 2, 3, and 4 in sequence) to form precipitates.
Then, the cobalt chloride solution was replaced with a freshly prepared solution containing equal volumes of a 6.25% (w/v) ammonium molybdate
solution and a 0.42% (w/v) ammonium vanadate solution.