The supplementation of urea or ammonium sulphate
(0.5 to 1.5%) as nitrogen source in the growth medium containing 2% wheat bran demonstrated that Urea enhanced GAAF production from 11 U/mL to 16 U/mL when used at a final concentration of 1.25% (Fig 3).
Purification of keratinase produced by the keratinolytic bacterial isolalates Isolates Purification Total Enzyme Spesific step protein activity activity (mg/ml) (U/ml) (U/mg) A4 Supernatant 55.41 108.70 1.96 Ammonium sulphate
(20%) 38.88 95.90 2.47 Dialysis 21.64 99.70 4.61 B4 Supernatant 52.18 65.80 1.26 Ammonium sulphate
(70%) 42.81 57.50 1.34 Dialysis 20.20 63.20 3.13 B6 Supernatant 66.79 86.80 1.30 Ammonium sulphate
(70%) 49.22 71.20 1.45 Dialysis 20.54 83.90 4.08 Isolates Purification Yield Purification step (%) (fold) A4 Supernatant 100 1 Ammonium sulphate
(20%) 88.22 1.26 Dialysis 91.72 2.35 B4 Supernatant 100 1 Ammonium sulphate
(70%) 87.39 1.07 Dialysis 96.05 2.48 B6 Supernatant 100 1 Ammonium sulphate
(70%) 82.03 1.11 Dialysis 96.66 3.14
(2013) obtained highest yield of cellulase enzyme using ammonium sulphate
as nitrogen source for bacteria isolated from soil.
Three different nitrogen sources were optimized and the enzyme produced with maize steep liquor was 3.89 IU/ml, yeast extract was 7.81 IU/ml and ammonium sulphate
had 11.57 U/ml.
The global, Far East and Baltic nations ammonium sulphate
market is segmented on the basis of production process and region.
Treatments consisted of control (weedy check), carfentrazone-ethyl + clodinafop-propargyl + metsulfuron-methyl @ 500, 375 g/ha alone and with 1% and 2% ammonium sulphate
1 bag ((NH4)2SO4) ammonium sulphate
may be applied on the crop when the time bulb formation takes place.
The growing of cotton roots are stimulated by the application of gypsum (Tanaka and Mascarenhas 2002) and by use of nitrogen in the form of ammonium sulphate
before the sowing (Carvalho 2007).
The precipitates obtained after fractionation at various saturation levels of ammonium sulphate
was then suspended in 25 mL of buffer B (Tris-HCl 0.02 M pH 7.3, NaCl 0.2 M, [CaChl.sub.2] 0.01 M), and dialysed in buffer solution C (Tris- HCl 0.01 M pH 7.3, NaCl 0.2 M, Ca[Cl.sub.2] 0.01 M) using selophan pocket (Sigma) until colorless buffer was obtained.
Effect of various Nitrogen sources like ammonium sulphate
, corn steep liquor (CSL), and urea were added to the growth medium to enhance growth and ethanol formation.
The unit said it would continue producing limestone ammonium nitrate, ammonium sulphate
and a range of ammonium nitrate and ammonium sulphate
based liquid and granular NPK fertiliser blends.
OCI said last month it had signed a deal with Egyptian Financial and Industrial Corporation (EFIC) to manufacture and sell 300,000 tons of ammonium sulphate