Inhibitory effects of anthraquinones
on bacterial collagenase., Yakugaku Zasshi 110, 688-692, 1990.
Thus, the light action in the presence of oxygen improved the antimicrobial effect of photosensitizing anthraquinones
by increasing the Redox imbalance, because of their ability to generate ROS, which resulted ultimately in an oxidative stress inside of C.
Chemical Analysis: Alkaloids, glycosides, starch grains, tannins, anthraquinone
, saponins and ferric chlorides were present whereas fixed and volatile oils were absent.
The GTNE and NNE was used to test the presence or absence of alkaloids, glycosides, sapoinins, flavonoids, steroids, anthraquinones
and tannic acid according to pre-established procedures21 including the measurement of secondary metabolites in crude form.
were not detected by TLC in the aerial parts.
Chrysophanol (CHR), a member of the anthraquinone
family, was originally extracted from plants of Rheum genus (Figure 2).
For these assessments, 0.05 mL aliquots of insect midgut homogenate (containing 1mg tissue equivalent) were added to 0.05 mL of homogenization buffer, without sucrose, containing 3.3 x [10.sup.-7] M [23,24-[sup.3][H.sub.2]]-E; 1.6 x [10.sup.-3] M NADPH; and one of the six anthraquinones
evaluated in concentrations from 1 x [10.sup.-8] to 1 x [10.sup.-3] M in the assay (assuming full anthraquinone
solubility in the assay medium and no selective partitioning into the membrane environment).
The preliminary phytochemical investigation of aqueous extract of the green tea showed the presence of alkaloids, flavonoids, steroids, tannins, proteins, amino acids, carbohydrates, reducing sugar, and deoxysugars and indicated the absence of terpenoids, saponins, glycosides, and anthraquinones
. The percentage yield of GTE was found to be 24.76%.
Test for anthraquinones
: An amount of 0.5 g of the test sample was heated with 10 (Percent) hydrochloric acid for some time in water bath and was filtrated, cooled and the same volume of chloroform was mixed.
were found only in the ethanol & aqueous extracts of leaf, and it is absent in the all the stem extracts.
Charcoal treatment is necessary since the latex, which exists as a separate liquid between the outer rind and inner fillet gel, contains bitter phenolic molecules including anthraquinone
C- and O-glycosides, anthrones, and some free anthraquinones