As basic structural unit of hypericin is emodin anthrone
, the search for possible biosynthetic routes for anthraquinone synthesis revealed the existence of an alternative pathway, through chorismate/o-succinylbenzoic acid pathway [61, 62].
Determination of protein-bound carbohydratebin serum by a modified anthrone
The WSC contents were determined by colorimetric after reaction with anthrone
reagent (Thomas, 1977).
(b) Total Starch content: The total starch content was determined by anthrone
Total carbohydrates were determined by spectrophotometric method, based on reaction between glucose and anthrone
, after hydrolysis.
Glycogen content of soft tissues was determined from April 2007 to January 2008 by the anthrone
method (Kamada & Hamada 1985).
The soluble sugar content was determined following the anthrone
method: 1 ml of a mixture of 0.2 % anthrone
in 100% [H.sub.2] S[O.sub.4] was added to 1 ml of sample and incubated in a 40[degrees]C water bath for 20 min.
At GS61 + 75[degrees]Cd (base temperature 0[degrees]C), percentage WSC content of stems and attached leaf sheaths was assessed in all plots, using the anthrone
method of Yemm and Willis (1954) as described by Gay et al.
The pellet was dried under nitrogen, then dissolved in 5 ml distilled water; the amount of glycogen in the solution was measured using the anthrone
method of Umbreit et al.
Glycogen contents were estimated in 30% potassium hydroxide solution (KOH) according to Anthrone
method of Consolazio and Lacono (1963).
Total soluble sugars were measured by anthrone
reagent (0.2%) following Sadasivam and Manickam (1992).
, and oyster-glycogen were procured from Himedia, Mumbai, India.