Moreover, results were interchangeable with immunoturbidimetric assays for apo A-I (Deming slope, 1.014) and for apo B-100
(Deming slope, 1.016) (1).
Total cholesterol, HDL-c, TG, Apolipoprotein A-1 (Apo A-1), Apolipoprotein B-100 (Apo B-100
) and glucose were measured in serum by standardized enzymatic automated methods in a PENTRA-400 autoanalyzer (ABX-Horiba Diagnostics, Montpellier, France).
The adopted purification procedure provided highly purified lipoprotein fractions as confirmed by the absence of plasma proteins as well as of apo B-100
in HDL (assessed by western blotting).
particularly the ratio between apolipoprotein B and apolipoprotein A-I (apoB/apoA-I).1819 The significance of LDL: Apo B-100
and HDL: Apo-A1 and their possible relationship with eclampsia is yet not clear.
Binding of LDLs to the LDLR is mediated by interactions between the LDLR and the Apo B-100
Study end points evaluated HbAlc, FSG, SMBG, lipids, Apo B-100
, adiponectin, fructosamine and glycated albumin.
We aimed to investigate the frequency of apo B-100
mutations (colon 3500) C9774T (Arg 3500 [right arrow] Trp) and G9775A (Arg 3500 [right arrow] Gln) in patients with atherosclerosis in comparison with healthy subjects.
This disorder can be caused by mutations in the gene encoding apo B-100
, leading to the formation of truncated apo Bs, which have a reduced capacity to export lipids from the hepatocytes as lipoprotein constituents.
and apo A-I levels are not readily available, so one could substitute LDL and HDL as best estimates.
Truncated apo B molecules shorter than apo B-70.5 are cleared from the plasma mostly by the kidney, whereas truncated apo B molecules with a size [greater than or equal to] 70% of that of apo B-100
are removed by the liver (1,2).
We used the monoclonal antibody 4E6, which is directed specifically against an epitope in the apolipoprotein B-100 (apo B-100
) moiety of oxLDL that is formed from the substitution of aldehydes for lysine residues in apo B-100