Moreover, results were interchangeable with immunoturbidimetric assays for apo A-I (Deming slope, 1.014) and for
apo B-100 (Deming slope, 1.016) (1).
Total cholesterol, HDL-c, TG, Apolipoprotein A-1 (Apo A-1), Apolipoprotein B-100 (
Apo B-100) and glucose were measured in serum by standardized enzymatic automated methods in a PENTRA-400 autoanalyzer (ABX-Horiba Diagnostics, Montpellier, France).
The adopted purification procedure provided highly purified lipoprotein fractions as confirmed by the absence of plasma proteins as well as of
apo B-100 in HDL (assessed by western blotting).
particularly the ratio between apolipoprotein B and apolipoprotein A-I (apoB/apoA-I).1819 The significance of LDL:
Apo B-100 and HDL: Apo-A1 and their possible relationship with eclampsia is yet not clear.
Binding of LDLs to the LDLR is mediated by interactions between the LDLR and the
Apo B-100 lipoprotein.
Study end points evaluated HbAlc, FSG, SMBG, lipids,
Apo B-100, adiponectin, fructosamine and glycated albumin.
We aimed to investigate the frequency of
apo B-100 mutations (colon 3500) C9774T (Arg 3500 [right arrow] Trp) and G9775A (Arg 3500 [right arrow] Gln) in patients with atherosclerosis in comparison with healthy subjects.
This disorder can be caused by mutations in the gene encoding
apo B-100, leading to the formation of truncated apo Bs, which have a reduced capacity to export lipids from the hepatocytes as lipoprotein constituents.
Apo B-100 and apo A-I levels are not readily available, so one could substitute LDL and HDL as best estimates.
Truncated apo B molecules shorter than apo B-70.5 are cleared from the plasma mostly by the kidney, whereas truncated apo B molecules with a size [greater than or equal to] 70% of that of
apo B-100 are removed by the liver (1,2).
We used the monoclonal antibody 4E6, which is directed specifically against an epitope in the apolipoprotein B-100 (
apo B-100) moiety of oxLDL that is formed from the substitution of aldehydes for lysine residues in
apo B-100.