A method to induce and collect
ascospores from Sclerotinia sclerotium of rapeseed in green house.
Ascospores 2-seriate, clavate, middle cells rounded, lower end cell long, 3-4 (-5-6) septate, slightly constricted at the septa, hyaline, smooth, 19-32 (-47.5) x 4.5-7 [micro]m ([bar.x] = 28.3 x 6.0 [micro]m; s= 5.0 x 0.8).
To enhance the perithecial and
ascospores development, the
ascospores were treated by adopting the method used by Yu (1954), which used 0.5% KOH solution for about 10 min.
Round
ascospores (arrowhead) inside the asci that are characteristic of S.
[8] suggested that
ascospores are dispersed in air currents rather than by rain splash, having important implications for risk-based decisions on disease control.
The powdery mildew epidemic begins when either
ascospores or conidia from flagshoots are dispersed from the bark or infected buds, respectively, and infect grape tissue.
Species of the order Pleosporales with applanate
ascospores can be found in three families (Diademaceae, Hysteriaceae, and Pleosporaceae), which differ in the way the ascomata open [1].
Molds commonly found in leaf surface/outdoor taxa (e.g., Basidiospores, 98%; Cladosporium, 97%;
Ascospores, 92%) were detected most frequently indoor and reflected what was detected outdoors and molds associated with severe water damage (e.g., Ulocladium, 18%; Chaeromium, 15%; Stachybotrys, 3%) were not as commonly detected indoors and also reflected what was detected outdoors.
Non-Viable Fungi (Spore) Air Sampling Results Spore Identifier Plenum Concentration (Spores/[m.sup.3]) Sample 1 Duplicate Sample algae - - Alternaria - -
ascospores 75 225 basidiospores 633 633 Botrytis - - Cercospora - - Cladosporium 83 142 clear brown - - Curvularia - - Curvularia-Xike - - Dechslera/Bipolaris group - - Epicoccum - - Helicosporium / Helicomyces - - hyphal elements 8 - Monochaetia - - Nigrospora - - Oidium - - Penicillium/Aspergillus 150 83 group Peronospora - - smuts, Periconia, - - myxomycetes Torula - - unknown - - Total 949 1083 Table 6.
