The conidial chain with isthimi consists of seven conidia and aggregates in slimy masses, single conidium smooth, gutullate, aseptate
, and the conidia remain attached to one another by narrow isthimi (Figures 3 and 4).
We also found similar fungal structures than the ones found in Gleichenia bifida (Willd.) Spreng by Schmid and Oberwinkler (1995): the endophytic fungal hyphae were aseptate
and spread inter- and intra-cellularly, they penetrated the host tissue through the rhizoids, developed vesicles in both the rhizoidal and the prothallic cells.
H & E stained sections of the biopsy showed an aggregates of broad, aseptate
hyphae with right angled, irregular branching (Figure 3) and a few rosettes of needle shaped crystals (Figure 4).
flagellate trichomes (or whip trichomes, Metcalfe & Chalk 1989): uniseriate, 2-3-celled with body differentiated in stalk and a long, whip-like terminal cell.
These fungi are often thought to be aseptate
, rather than pauciseptate.
Two cases of pigmented fungi were identified both of which presented as frontal abscesses, which had dark-pigmented hyphae and two cases of zygomycosis that were characterized by aseptate
The septation of the macroconidia was 3 to 5 and the microconidia were usually aseptate
or single septate.
The colonies on SDA were fluffy to cotton white at first, becoming grayish brown, spreading widely by means of aseptate
stolons (completely filling the Petri-dishes within 36-48 hours).
The diagnosis was established by direct microscopic evidence of broad, aseptate
or sparsely septate ribbon-like hyphae with right angle branching in KOH wet mount and histopathological examination of stained sections.
A Grocott-Gomori methenamine--silver nitrate stain demonstrated broad aseptate
hyphae with obtuse-angle branching.
These spaces contain masses of intermingled, aseptate
fungal hyphae, up to 6 [micron]m thick, frequently producing vesicle-like bladders (Fig.
Histopathological studies reveal that in tissue Mucorales hyphae can be distinguished from common opportunistic moulds by their broad (3-25 pm) diameter thin walled, mostly aseptate
hyphae together with angio-invasion.