Several authors disclosed ties to Danone Research, which partially funded the study; Thermo Fisher Scientific provided the instrumentation assay reagent
and disposables for the analysis.
It's not hard to imagine this scenario arising, for instance, due to degradation of an assay reagent
, and to see how the use of control charts would allow the detection of this trend, possibly even before any significant impacts on assay sensitivity have occurred.
RPMI 1640 (Gibico, USA), fetal bovine serum (FBS) (Hyclone, USA), antibiotics (North China pharmaceutical Group Corporation, China) cell-lysis buffer (Applygen Technologies Inc., China), bicinchoninic acid assay (BCA) protein assay reagent
(Pierce, USA) were applied in the research.
INR responses vary widely depending on the thromboplastin assay reagent
, but a calibrated anti-Xa assay appears to be a suitable method for determination of Riva concentration (13-16).
The HaemosIL assay reagent
kit contained 2 vials of 4.5mL of reagent with the cost per test estimated to be NZ$9.10.
Protein concentration of supernatant was measured with Bio-Rad protein assay reagent
. Cellular proteins were separated by 10% SDSPAGE and transferred to a polyvinylidene difluoride membrane.
With the Guava ViaCount Flex assay reagent
, quick and reproducible absolute cell count and viability assessments can now be done with insect and other non-mammalian cells, announced Guava Technologies, Inc., Hayward, Calif.
Unfortunately, automation cannot address the concern of assay variability that points to the heterogeneity of the allergen-containing assay reagent
. Differences in the composition of the allergen-containing reagents of the same specificity between assays is believed to be the primary contributor to the differences that are observed in final reported allergen-specific IgE antibody levels between assay methods.
We added 100 [micro]L luciferase assay reagent
(Promega) to supernatant (10 [micro]L) at 25 [degrees] C, and measured luciferase activity in a luminometer (Monolight 2010; Analytical Luminescence Laboratory, Ann Arbor, MI).
All of these systems feature easy-to-use assay reagent
packs, software and supplies.
The luminometer was programmed to inject each well with 20 [micro]L of Luciferase Assay Reagent
(Promega, Madison, WI), and read the luminescence generated for 10 sec.