As the attenuated and activated isolates were taken from axenic culture
, the differences found in the strain invading tissues can reveal new molecules involved in amoebic pathogenicity, in addition to confirmation of virulence genes found by other authors.
Fungi and bacteria-free plates were transferred to axenic culture
by placing the amoebae in PYG medium as previously described for further morphological and molecular analyses [17, 25, 26].
Temperature regulation of extracellular proteases in ectomycorrhizal fungi (Hebeloma spp.) grown in axenic culture
For the present study already established axenic culture
of Stylonychia mytilus in this laboratory was used.
A strain of Microcystis aeruginosa Kutzing was isolated from the Barra Bonita reservoir and is currently maintained as an axenic culture
(BB05) in the algal culture collection at the Botany Department of the Federal University of Sao Carlos (SP, Brazil) (World Data Center for Microorganisms 835).
Its stock clonal axenic culture
has been maintained on agar slants (1% v/v) at 28 [+ or -] 2[degrees]C, illuminated with 1500 lux of incandescent light, culture was grown and maintained in B[G.sup.11] medium  has been used as the basal medium in all experiments.
This two-membered assemblage was isolated from a brackish marsh located in Woods Hole, Massachusetts, by capturing a single Anabaena filament with attached epibiotic cells, serially washing the filament in sterile autotrophic medium (25% SO) to remove any contaminating bacteria, and using the washed filament to inoculate an axenic culture
of the Anabaena sp.
histolytica samples (HK9 strain) stored in liquid nitrogen were thawed and grown in axenic culture
in culture flasks using TYI-S33 culture medium at 37[degrees]C for 3-4 days, yielding only trophozoites .
Because the particles were assumed to be bacterial pathogens, efforts were made to produce an axenic culture
of the ameba isolate, and 16S rRNA PCR was performed to identify any intracellular bacteria.
The axenic culture
medium 199 supplemented by 5% of calf newborn serum was used.
The PLY429 culture was an axenic culture
, whereas the cyanobacterium for this experiment was isolated from a greenhouse culture of PLY429, where it had appeared periodically as a contaminant.
Aquatic plants stimulate the growth of and biofilm formation by Mycobacterium ulcerans in axenic culture
and harbor these bacteria in the environment.