In BOD test it was used OTC and CIP as sole carbon sources (Table 2).
Quantification in samples from BOD test and from Soil respiration test were done by high pressure liquid chromatography (HPLC) with UV-visible detector, Kromasil[R] 100-5C18 column with 15 cm / 4.6 mm (5 [micro]m), Teknokroma[R] TR-C-160-1 (ODS) precolumn and 1 mL/min flow mobile phase.
A major disadvantage of the BOD test
is the amount of time (5 days) required to obtain the results.
The samples were diluted using buffered dilution water, measured for dissolved oxygen and incubated at 20 [degrees] C (68 [degrees] F) for five days in a standard BOD test
. The results showed that dilutions of 0.05% and 0.1% of the original sample were appropriate for this case.
The BOD test thus represents a microcosm of the larger world we inhabit, and if we understand or measure that microcosm with only limited success, perhaps that's just a further reflection of our incomplete understanding of the world outside.
All of which makes the BOD test the perfect environmental counterpart to the ancient art of alchemy.
In automating the BOD test
, laboratories eliminate operator-to-operator variability, reduce operator involvement, and accurately control all process timing steps.
Other Hach devices include a system for running BOD tests
and downloading the stored data to a desktop computer.