The last 0.025 mL was discarded and 0.025 mL of antigen containing 4 HAU was added to all the wells except row H which serves as back titration.
Back titration was carried out; thus, 0.025 mL of antigen suspension containing 4 HAU was added into each of the first two wells of row H (4 HAU control from H1-H6), and twofold dilution was made from H2 to the H6 and the last 0.025 mL was discarded in order to obtain 4, 2, 1, 0.5, 0.25, and 0.25 HAU.
For example, if soils had a [pH.sub.KCl] of [less than or equal to ] <6.5, then acid-neutralising capacity by back titration
(ANCBT) and excess ANC ([ANC.sub.E]) were not attempted.
2.1 Determination of %age Lime Contents: Percentage lime contents were determined by back titration method with the standards solution of alkali (NaOH).
2.2 Determination of %age Organic Matter: The organic matter in the sample was determined by back titration. 1g of soil sample is taken in the titration flask along with 10ml of 1N Potassium dichromate, 20 ml of sulphuric acid (98.5%) and allowed it to cool for one hour.
the 795 KFT Titrino offers the following additional possibilities: Method memory for over 100 operator methods; Sample data storage for automatic sample processing using sample changers with up to 156 sample data records; Alphanumerical input of method names, sample identifiers and result units or descriptions; Connection of a 776/765 Dosimat for automatic titer determination or automated back titrations
; Connection for KF oven and 774 Oven Sample Processor; Barcode reader and PC keyboard can be connected (options); and Printout conforming to GLP with result, titer, date and instrument serial number.