Baculovirus


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Baculovirus

[‚bak·yə·lə′vī·rəs]
(virology)
A genus of invertebrate viruses that contains enveloped, double-stranded, supercoiled deoxyribonucleic, includes the subgroups nuclear polyhedrosis virus and granulosis virus.
References in periodicals archive ?
Se observaron sintomas tipicos de infeccion por baculovirus en larvas de Chrysodeixis includens Walker (Lepidoptera: Noctuidae) criadas en laboratorio en Tucuman, Argentina.
The recombinant baculovirus was subcultured in Sf9 cells to the second passage (titer of approximately [10.sup.7] pfu/mL) and then stored at 4[degrees]C for later use.
The baculovirus delivery system enables consistent expression in a wide variety of cell types.
NPV Second, DnNPV baculovirus particles exhibited truncated ends, and rounded ends were observed in NezhNPV virions.
The deleted mutants of this protein expressed in baculovirus system allowed us to localize the amino acid sequence of the truncated N-protein of PPRV between 1-420 to be responsible for inducing immune suppression like N-protein of other Morbilliviruses.
The recombinant bacmid was then transfected into Sf-9 cells to generate the baculovirus. The Per a 5 protein was expressed in Sf9 cells and purified by Ni column.
Baculovirus is an excellent tool to overexpress recombinant proteins in insect cells.
The collaboration between Avalanche and Lonza is expected to focus on the development and high-yield AAV production based on a novel technology that uses a stable baculovirus expression system to produce AAV vectors in insect cells under serum-free conditions.
El Baculovirus penaei fue analizado utilizando un volumen de reaccion de 20 [micron]L, constituido por 2 [micron]L de buffer Taq 10X, 1,5 mM de Mg[Cl.sub.2], 0,2 mM de cada dNTPs, 1 U de Taq ADN polimerasa recombinante, 5 pmol de cada cebador y 2 [micron]L de ADN extraido.
Algunos ejemplos para el control de la enfermedad de Gumboro incluyen: proteina VP2 expresada en levaduras [17], en el sistema baculovirus [7] y vacunas recombinantes del virus de la enfermedad de Newcastle [14].
Automating these tasks allows scientists to maintain cultures of stock cells continually and automate infection for the production of baculovirus and protein reagents.

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