longa [5,6] Chemical Method Result substance tested Alkaloid Mayer's test + Dragendorff's test + Hagers test + Wagner's test + Tannins present Ferric chloride test + Gelatin test + Lead acetate test + Flavonoids Ferric chloride test + Magnesium hydrochloride test + Sodium hydroxide test + Mineral acid test + Carbohydrates Molisch's test + Benedict's test + Fehling's test + Barfoed's test
- Phytosterols Liebermann-burchard test + Proteins and amino Millions test - acids Biuret test - Ninhydrin test - Lactones Acetic anhydride test + Alkaline nitroprusside test + Triterpenes Salkowski test + Liebermann-storch mora sky + test Hirshhorn test + Anthraquinone Borntrager's test + glycosides C.
Barfoed's Test: 1 ml of extract of the plant material was taken in a test tube.
Test for Carbohydrate * Molisch's test + + + * Fehling's test + [+ or -] - * Barfoed's test + - - 3.
Test for carbohydrate through Molisch's test indicated their presence in chloroform extract as well as rectified spirit extract, but the results were somewhat variable in Fehling's and Barfoed's tests. Presence of glycosides was observed in all five plant chloroform extracts but not in rectified spirit extracts.
After hydrolysis, the presence of carbohydrates in the hydrolyzed samples was investigated by different biochemical tests including Molisch Test, Iodine Test, Benedict's Test, Tollen Test, Barfoed's Test, Fehling's solution Test, Osazone Test, Seliwanoff's Test and Bial's Test.
A number of biochemical tests including Fehling's Solution Test, Benedict's test , Combur Test , Molisch Test, Iodine Test , Tollen Test, Barfoed's Test, Osazone Test and Seliwanoff's Test were performed by using the reported methods (Fig.
A number of chemical tests including Molisch Test, Iodine Test, Benedict's Test, Tollen Test, Barfoed's Test, Fehling's solution Test, Osazone Test, Hydrolysis Test and Seliwanoff's Test have been found to be positive and strongly support for the presence of reducing sugars and other carbohydrates in the water extracts of M.