We observed inverse (protective) associations between ASD diagnosis and several air toxics: 1,4-dichlorobenzene, 4,4'-methylene diphenyl diisocyanate (MDI), benzidine
, and ethyl carbamate (urethane) and for 1,4-dichlorobenzene with autism severity.
Results and Discussion Synthesis of Dyes 6a-j Synthesis of Congo red dye and its derivatives (6a-j) was achieved in excellent yields, according to the synthetic strategy outlined in scheme 1 starting from benzidine
** Worked at work or home with asbestos, arsenic salts, chromium salts, cadmium salts, asphalt, mineral oils, benzidine
, benzene, isopropyl oil, dyestuffs, vinyl chloride, pesticides, herbicides, mustard gas, welding, and wood dust.
Next, the enzyme substrate (3,3',5,5'-tetramethyl benzidine
peroxidase substrate and peroxidase solution B) was added, and the samples were incubated for 15 min (IgM) or 30 min (IgG).
Each sample was analyzed in duplicate using, peroxidase as the enzymatic tracer, a polyclonal antibody (L2) and tetramethyl benzidine
as color reagent (L2 antibodies have a six-to eightfold higher affinity for ecdysone than for 20E).
A 50 [micro]l aliquot of the supernatant was added to the reaction mixture consisting of 1.6 mM tetramethyl benzidine
and 0.1 mM H202, incubated at 37[degrees]C for 3 min, and then monitored the absorbance at 650 nm.
In this assay, performed in duplicate, the following was added to every 20 [micro]l of homogenate: 60 [micro]l of 90 mM potassium phosphate buffer (final pH adjusted to 7.2), 200 [micro]l of Na acetate/TMBZ working solution (0.012g of 3,3,5,5 tetramethyl benzidine
dihydrochloride in 6 ml of methanol and 18 ml of 250 mM sodium acetate buffer at pH 5.0), and 25 [micro]l of 3% hydrogen peroxide ([H.sub.2][O.sub.2]).
Excess HRP-conjugate is washed off and solution of tetra methyl benzidine
(TMP) reagent was added.
In brief, 10 [micro]L of Hb-enriched plasma was subjected to electrophoresis in a nondenaturing gel, and the gel was subsequently immersed in solution containing a congener of benzidine
with a precipitate forming in the gel corresponding to the location of Hb-Hp complexes.
I noticed that one of the starting materials for the API resembled benzidine
(4,4'-diaminobiphenyl), a known carcinogen.
Antigenantibody complex was visualized by 3.3'--diamino benzidine
and 0.03% of [H.sub.2][O.sub.2].
Govindwar 2007.Biodegradation of benzidine
based dye Direct Blue-6 byPseudomonas desmolyticum NCIM 2112.