fluid sample and 10 [micro]l of the spent media sample were each subjected to a modified version of MDA (Qiagen, UK) using a protocol developed at Reprogenetics specifically for the purpose of amplifying DNA from blastocoel
fluid and spent media samples (Babariya and Wells, unpublished).
Skeletogenesis thus resembled development of typical feeding larvae, in which these spicules are secreted by a mass of primary mesenchyme cells that migrate into the blastocoel
shortly before the start of gastrulation (Okazaki 1975).
At the blastocyst stage, however, sodium pumping on the part of the trophoblast results, through osmosis, in the drawing of water into the blastocoel
, enlarging the blastocyst within the zone pellucida.
At 9 h after spawning, embryos consisted of a bilayer of cells with no definite blastocoel
. The embryos then became concave-convex dish-shaped (Fig.
Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel
formation and hatching of in vitro and IVM/IVF derived porcine embryos developing in vitro.
Previous lectin-staining results demonstrated that the mesh-like structure of the extracellular matrix included acid mucopolysaccharides in the blastocoel
of the archenteron-forming P.
As the eggs have abundant small lipids, the blastomeres release lipids into the blastocoel
, causing an increase in cell number at the wrinkled-blastula stage and the embryonic surface becomes smooth (1).
, which first becomes apparent in the 8-cell stage, is very pronounced beginning with the 32-cell stage (Fig.
Serotonin stimulates [[Ca.sup.2+].sub.i] elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel
The ingression of cells into the blastocoel
at gastrulation requires those cells to undergo EMT, making the regulation of cell adhesion crucial for the normal development of the organism.
The cells stained weakly with P4 are seen in the blastocoel
of the six-armed pluteus (Fig.
During the gastrula stage, lipid droplets were extruded into the blastocoel