All of the 3PN embryos could be achieved at blastocyst
stage on day 5 or 6.
"Our findings confirm that interspecific blastocyst
complementation is a viable method for kidney generation," said researcher Masumi Hirabayashi.
Researchers take blastocysts
, the clusters of cells formed several days after egg fertilisation, from mutant animals missing specific organs and inject them with stem cells from a normal donor, not necessarily of the same species.
Currently, evaluating blastocyst
morphology and preimplantation genetic screening/diagnosis (PGS/PGD) are the two methods used to select healthy embryos for implantation.
Then, the embryos (5 blastocyst
: day 7) were placed in an Eppendorf tube containing 5 ul of 0.1% PBS-PVA and stored at -80AdegC (three replicates in each group) until RNA extraction.
Determination of diameter and quality of blastocyst
There was no difference between reactions of fresh and frozen groups in both 2-cell and blastocyst
Numerous previous reports have shown that higher pregnancy rates have been observed with blastocyst
transfer (Day 5) than with transfers of early cleavage stage (Day 2 or Day 3) embryos [12-14].
rate with LP-PDMS was similar to those of the group culture (control) and WOW (32% versus 38% and 15%, respectively; P>0.05).
It is possible to establish three different types of stem cells, including ES cells, from thes three cell types in the blastocyst
Over the next several days, certain mitotic divisions take place until blastocyst
formation, but these events are unsuitable for observations, as they occur in the oviducts and the uterus.