tribuloides (Wray and McClay, 1988), blastomeres
were size-fractionated using a sucrose gradient, then micromeres from donor embryos were combined with host blastomeres
to form chimeras.
On the other hand, blastocyst stage embryo has also the advantage of possessing many cells, thus the loss of few blastomeres
during the freezing and thawing processes might not compromise the integrity of the entire specimen.
The researchers obtained forty-eight blastomeres
from the seventeen polyspermic embryos (eight two-cell, two three-cell, five four-cell, and two eight-cell), or theoretically forty-eight new totipotent embryos.
fomicata, the polar lobe and individual blastomeres
may be isolated to study subsequent development and to assay for developmental potential and the presence of specific cell-cell inductive interactions (see section F1 in Henry et al.
For the second cleavage, the PL extruded from the large blastomere
(data not shown).
morphology as a predictive measure of fish egg viabilility.
The means of fixation of the mutual positions among the blastomeres
of sea urchin larvae.
Transfer of mtDNA even in the form of blastomere
nuclei (the technique which has been scrutinised the most by ethicists) is not 'reproductive cloning of a human being'.
To construct the parental haplotype, we performed All-in-One target sequencing using genomic DNA extracted from the peripheral blood of parents (PED-Mother & PED-Father) and the proband daughter (PED-Sister) as well as WGA products from the 7 blastomeres
, including the transferred embryo (PED-E-B) and the other 6 embryos nontransferred (PED-E-1,2,4,5,6,7).
Epidermal cells are differentiated, even if blastomeres
are continuously dissociated to prevent cell--cell interactions until the early gastrula stage.
Fluorescence in situ hybridization analysis of two blastomeres
from day 3 frozen-thawed embryos followed by analysis of the remaining embryo on day 5.
In fact, the Oct-4 is a protein of Pit-Oct-Unc family (5) which is characterized by exclusive expression in blastomeres
, in the inner cell mass of the blastocyst, and in the germ cell lineage (6), (7).