Uneyed embryos were stained for 5 min in Bouin's solution
to enable staging (Stevens, 2006).
Biopsy was sent to the pathology laboratory in Bouin's solution
Also, a piece of the biopsy specimen was placed in a tube which contained Bouin's solution
to fix the specimen for pathological investigation
Samples of tissues were also placed in cassettes and fixed in Bouin's solution
for later histological processing (see Shields et al.
Testes were removed and fixed in Bouin's solution
, embedded in paraffin an stained with Hematoxilina and Eosin Y.
Firstly, the boiling shrimp sample was fixed to preserve its tissues in Bouin's solution
Male gonad segments were fixed in Bouin's solution
for 6 hours and embedded in paraffin, longitudinal sections (5mm) were processed for histology using the hematoxyline/eosin method.
ocellata tissues fixed in Bouin's solution
and stained with toluidine blue (0.
After 30 min, cells were washed with phosphate-buffered saline (PBS) and fixed with Bouin's solution
for 5 min.
The ovaries were removed, fixed with Bouin's solution
, dehydrated in a graded ethanol-n-butanol series, embedded in paraffin and serially sectioned at 5 [micro]m thickness.
For gonadal histology, 30 fish were fixed in toto for 24 hr in Bouin's solution
and stored in 70% industrial methylated spirits (IMS) before processing for histology.
The edible part was removed from the shell, weighed to the nearest mg, fixed in Bouin's solution
for 72 h, preserved in 70% alcohol, dehydrated in ethanol series and infiltrated with paraffin.