The alkaline distillate was titrated against 0.1 M HCl using bromocresol green
and methyl red indicators.
Peripheral blood was drawn from all the study subjects under aseptic biosafety precautions Albumin measured in serum (yellow-top tube) AND method used is bromocresol green
Olympus AU2700 analyser.
Folin-Ciocalteu reagent, bromocresol green
were purchased from Sigma-Aldrich, St.
Biochemical parameters included Hemoglobin estimated through Sysmax K-21 Hematology analyzer, Serum albumin employing Bromocresol Green
Method (Cheesbrough, 2005), protein using Biuret method (Rose, 1833) and calcium through Clark and Collip method (Cheesbrough, 2005), was estimated using Human kits.
They included bromocresol green
, bromophenol blue, bromothymol blue, bromocresol purple, bromocresol blue, cresol red, cresol blue, cresol purple (BDH Chemicals Ltd., Poole, England); Diaverdine (Elnaser pharmaceutical chemicals Cairo, Egypt); and New-Cox powerful anticoccidial watersoluble powder (Atco Pharma for Pharmaceutical Industry, Cairo, Egypt).
(BCG) solution (200 [micro]g/mL) was prepared by dissolving BCG in 10 mL ethanol and diluted to 100 mL with distilled water, while a buffer solution was prepared from potassium hydrogen phthalate in water.
Diethanolamine (DEAM), tetrahydrofuran (THF), N,N-dimethylformamide (DMF), 2,2'-azobisisobutyronitrile (AIBN), ethyl alcohol (Fluka), n-hexane, 1,4dioxane, dichloromethane, sodium bicarbonate (NaHC[O.sub.3]), sodium hydroxide (NaOH), and bromocresol green
(BCG) were used as received without any further purification.
Each well of ELIZA plates was filled with 990 [micro]l of MRS broth with Bromocresol Green
(0.04 gm/1000 ml) with pH range of 3.8-5.4.
Serum calcium was calculated using Biotech 3000 Analyzer using cresolphthalein method, and serum albumin was calculated using bromocresol green
Eight hundred consecutive serum specimens received in the laboratory with a request for calcium estimation were analyzed for calcium and albumin in Erba XL 640 AutoAnalyzer by Arsenazo III method for calcium and bromocresol green
in succinic acid buffer at pH 4.2 for albumin.
In an effort to understand the extent of impurities in these dyes and the potential impact on the expanded uncertainty of spectrophotometric pH measurements, we have examined seven commercially available sulfonephthalein pH indicators using liquid chromatography-mass spectrometry (LC-MS): bromocresol green
(BCG), bromocresol purple (BCP), bromothymol blue (BTB), cresol red (CR), meta-cresol purple (MCP), phenol red (PR), and thymol blue (TB).