Eduard Buchner

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Buchner, Eduard

(ā`do͞oärt bo͞okh`nər), 1860–1917, German chemist. He taught at Berlin, Breslau, and, from 1911, at Würzburg. He discovered (1896) that alcoholic fermentation of sugars is caused by yeast enzymes and not by the yeast cells themselves. Zymase, part of the enzyme system causing fermentation, was discovered by him in 1903. For this work he received the 1907 Nobel Prize in Chemistry.
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The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.

Buchner, Eduard

 

Born May 20, 1860, in Munich; died Aug. 13, 1917, in Focşani, Rumania. German chemist; worked with A. Baeyer.

From 1893 to 1911, Buchner was a professor in Kiel, Berlin, Breslau (Wrocńaw), and Würzburg. He discovered pyrazole in 1889. In 1897, using compression, he obtained from yeast a juice that contains no living cells but is able to stimulate vigorous fermentation. In this same way a juice that stimulates lactic and acetic fermentation was later obtained from other organisms. These works showed that fermentation can take place without the participation of lower organisms. Buchner won the Nobel Prize in 1907.

REFERENCE

Hjelt, E. Istoriia organicheskoi khimii s drevneishikh vremen do nastoiashchego vremeni. Kiev-Kharkov, 1937. (Translated from German.)
The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.
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After 5 days of cultivation on a rotary shaker at 180 rpm under 28 [+ or -] 1[degrees]C, the mycelia were separated by filtration using a Buchner funnel. Then the mycelia were drained and freeze-dried at -54[degrees]C and under 0.5 mbar vacuum for 12 h.
Vacuum filtration setup (much preferred): Buchner funnel (7 cm or larger) Filter paper (7 cm or larger) Filter flask (125 mL or larger) Vacuum adapter Vacuum tubing Water aspirator or Gravity Filtration setup (possible but not recommended): 7 cm funnel Larger filter paper, folded into a cone (cotton ball inadequate) Erlenmeyer flask (125 mL)
Deionised water was ponded to a depth of 20 mm on top of a 3-[micro]m Millipore paper in a Buchner funnel. A 5-mm-diameter air-dry aggregate of soil was placed into the deionised water and left for 2 h and subsequently treated according to the SAWM.