The mentioned enzyme is a part of the Ser/Thr protein kinase family, whose function is to phosphorylate a hydroxyl (-OH) group of serine or threonine in proteins with the help of cAMP-dependent protein kinase
which activates it through phosphorylation [6, 7].
The MOD_PKA_2 motifs matched with amino acid residues CRVSGGE (279-285) that is part of a functional residue of secondary preference for PKA-type AGC kinase phosphorylation in cytosol, nucleus, and cAMP-dependent protein kinase
Ahn, "Activation of presynaptic camp-dependent protein kinase
is required for induction of cerebellar long-term potentiation," Journal of Neuroscience, vol.
Recently, genetic defects in cAMP-dependent protein kinase
type 1-[alpha] regulatory subunit (PRKAR1A) and cAMP-specific phosphodiesterase 4D (PDE4D) were identified in patients with acrodysostosis by candidate gene analysis (4) and exome sequencing (2,5) respectively.
Kopperud et al., "Epac1 and cAMP-dependent protein kinase
holoenzyme have similar cAMP affinity, but their cAMP domains have distinct structural features and cyclic nucleotide recognition," Journal of Biological Chemistry, vol.
Characterization of agonist stimulation of cAMP-dependent protein kinase
and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies.
PKA, also called cAMP-dependent protein kinase
A, is organized as tetramers comprising two catalytic and two regulatory subunits.
For positive control along with reaction mixture dilutions (using PKA dilution buffer- 380 mM K3PO4 andmM DTT) of cAMP-dependent protein kinase
in variable concentrations (0.5, 2.0, 4, 6.0 and 8.0 [micro]l) were added accordingly to make final volume of 25 [micro]l.
Goodman, "The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase
A," The Journal of Biological Chemistry, vol.
It has been suggested that cAMP-dependent protein kinase
(PKA) signal pathway was involved in dopamine-mediated change of [Na.sup.+], [K.sup.+]-ATPase activity after short-term or long-term morphine treatment .
Adenylyl cyclase transforms ATP into cyclic AMP(cAMP), which in turn increases the activity level of cAMP-dependent protein kinase
A (PKA) [3, 4].
Clark, "Characterization of agonist stimulation of cAMP-dependent protein kinase
and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies," Molecular Pharmacology, vol.