, BDNF, NGF, synapsin, FGF2 gen ekspresyon seviyelerinin degisimi, kontrol grubu ile karsilastirilarak hesaplandi.
To analyze CREB
and pCREB levels, the data obtained from each band were normalized by the respective [alpha]-tubulin value.
Among the signal transduction pathways in pituitary gonadotrophs, the PKC-MAPK pathway primarily activates c-Jun, Elk-1, and Egr-1, and the cAMP-PKA pathway primarily activates CREB
and Egr-1, while the Ca2+-mediated signaling pathway primarily activates Nur77, c-Jun, and Egr-1 (Naor et al., 1997; Melamed et al., 2012; Thompson et al., 2013).
Various chronic stressors such as chronic social defeat can reduce CREB
or pCREB and BDNF expression in the PFC [52-54], though studies do not show uniform results [55, 56] due to differences in experimental conditions, such as stress paradigms, brain areas analyzed, and time of testing.
Although several studies suggest a key role of CREB
in DC maturation and function, a detailed lack of function study in vivo is still missing.
Semiquantification of CREB
and Flk-1 by immunofluorescence was conducted with ImageJ (version 1.50i software; ImageJ, National Institutes of Health, Bethesda, MD, USA).
Downregulation of the Expression of DR6, ERK, and CREB
Paricalcitol Increases Phosphorylation of Akt and CREB
via EP4 in IR-Exposed HK-2 Cells.
Ultimately, ERK signal cascades activate CREB
gene expression which is thought to be responsible for neurogenesis and neural development [47,48].
The relative value of the mRNA expression of CREB
genes was calculated by comparing the cycle thresholds (CTs) of the target gene with the housekeeping gene (GAPDH) using the [2.sup.-[DELTA][DELTA]ct] method.
[sup] Membranes were probed with primary antibodies against CREB
, phospho-Ser133-CREB (#9104 and #9191, respectively, Cell Signaling, USA), Arc , c-fos , and Egr1 (ab118929, ab53036, and ab55160, respectively, Abcam, USA).
Phosphorylation of CREB
and mechanical hyperalgesia is reversed by blockade of the cAMP pathway in a time-dependent manner after repeated intramuscular acid injections.