Calibration curves with caffeic acid
(0-25 ppm) were created and the final results were expressed in terms of caffeic acid
(CA) equivalents per gram of DW (mg/g CA DW).
phenethyl ester modulates methotrexate-induced oxidative stress in testes of rat.
-A number of potentially clinically relevant compounds are present in coffee, including: caffeine, hydroxycinnamic acids notably chlorogenic acid, trigonelline, diterpenes eg cafestol and kahweol, and caffeic acid
( ANI )
HPLC fingerprinting of extracts revealed the presence ofthe gallic acid ([t.sub.R] =11.67 min; peak 1), chlorogenic acid ([t.sub.R] = 21.45 min; peak 2), caffeic acid
([t.sub.R] = 24.08 min; peak 3), coumarin ([t.sub.R] = 30.21; peak 4), rutin ([t.sub.R] = 38.19 min; peak 5), quercitrin ([t.sub.R] = 46.57 min; peak 6), quercetin ([t.sub.R] = 49.97 min; peak 7) and kaempferol ([t.sub.R] = 52.11 min; peak 8) (Fig.
The following phenolic compounds were identified in the extracts: chlorogenic acid, caffeic acid
and rutin (Table 2), and the retention time were 10.8, 11.5 and 14 minutes (Figure 1), respectively.
The total amounts of 6 phenolic acids (t-cinnamic acid, benzoic acid, p-coumaric acid, caffeic acid
, ferulic acid, and chlorogenic acid) were 1.58-fold lower in the roots compared to the aerial parts.
Five phenolic compounds were identified and quantified, namely, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid
, and p-coumaric acid.
The most common HCA derivatives include caffeic acid
, ferulic acid, and p-coumaric acid, to name a few.
For quantification of phenylethanoid derivatives, caffeic acid
(20 ng/[micro]L) was used to determine the relative concentration of this chemical group by LC-PDA (320 nm), and the identification of these compounds was confirmed by ESI-MS/MS.
Stock standard solutions were prepared by accurately weighing 10 mg of caffeic acid
, chlorogenic acid, p-coumaric acid, trans-ferulic acid, cinnamic acid, quercetin, luteolin, apigenin, rutin, vanillic acid, syringic acid, o-coumaric acid, catechin, vanillin, trans-chalcone, 4-hydroxybenzoic acid, 2-(4-hydroxyphenyl)ethanol, and 3,4-dihydropyran reference standards into separate 2 ml volumetric flasks and dissolving in methanol.
Green coffee beans contain polyphenol compounds, such as chlorogenic acid and its related compounds (caffeic acid
, ferulic acid, and coumaric acid) which are used to inhibit the oxidative stress .
We show that CGA and caffeic acid
displayed a dramatic protective effect against the nitric oxide donor, sodium nitroprusside.