The carboxyl terminal
portion having four zinger motifs forming a domain for DNA binding and regulates the early growth response gene 1 family i.e.
Most strategies focus on the M protein, a surface protein with a high changeable amino terminal and a conserved carboxyl terminal
On immunohistochemistry, ASPS is consistently positive for an antibody that detects carboxyl terminal
of transcription factor 3 (TFE3) gene in the form of strong nuclear staining pattern [23, 24].
Rhoassociated kinase (ROCK) is a small Rho-binding protein that belongs to the Rho family with protein kinase activity of threonine/serine along with a molecular mass of nearly 160 kDa [38-41], and a pleckstrin homology (PH) domain rich in cysteine at the carboxyl terminal
of the protein.
The 1125 bp DNA encoding the carboxyl terminal
of [MSP1.sub.42]kDa gene from P falciparum (Indian isolates) was amplified from the parasite genomic DNA using high fidelity Pfu polymerase (Fermentas, USA) employing the following primers having introduced SnaB I and Not I sites (bold ad underlined sequences) in the forward and reverse primers respectively.
When no binding hormone is present the carboxyl terminal
Abbreviations: ADP: adenosine diphosphate; AMPPNP: [beta],[gamma]-imidoadenosine 5'-triphosphate; ATP: adenosine triphosphate; BFP: blue fluorescent protein; COOH-terminal: carboxyl-terminal; C-domain: carboxyl terminal
domain; EGTA: ethylene glycol tetraacetic acid; GFP: green fluorescent protein; N-domain: aminoterminal domain; NH-terminal: amino-terminal; Pi: inorganic phosphate; S1: subfragment 1 of myosin; TnC: troponin-C; TnI: troponin-I; TnT: troponin-T; Tn[T.sub.1]: troponin-[T.sub.1]; Tn[T.sub.2]: troponin[T.sub.2]; Tn-(I+C): binary complex of Tnl and TnC; Tn-([T.sub.2]+I+C): ternary complex of Tn[T.sub.2], TnI, and TnC; Vi: vanadate.
Second generation immunoassays, called intact PTH analysis, have a system of two antibodies, one specifically for the carboxyl terminal
portion and one for the amino terminal portion of the molecule.