References in periodicals archive ?
The exceptions were extracellular proteins, such as serine carboxypeptidase, thaumatin-like protein, acid [alpha]- and [beta]-galactosidase, peroxidase, as well as [alpha]-xylosidase.
Determination of pancreatic carboxypeptidase A in human blood serum.
Measuring carboxypeptidase A activity with a centrifugal analyzer: analytical and clinical considerations.
A unique activity assay for carboxypeptidase A in human serum.
Determination of carboxypeptidase A using N-acetyl-phenylalanyl-3-thiaphenylalanine as substrate: application to a direct serum assay.
Carboxypeptidase U (CPU, (1) activated thrombin-activable fibrinolysis inhibitor; EC 3.
Another important advantage of activity assays compared with antigen assays is that the 2 described polymorphisms in the coding region of proCPU (Thr147A1a and Thr325I1e) have similar activation kinetics and the activated enzymes have similar carboxypeptidase B-like activity toward small synthetic substrates (26, 28).
Typical assays for carboxypeptidase activity rely on a change of absorbance, and sensitivity is always an important issue.
The most important advantage of activity assays compared with antigen assays is that the 2 described polymorphisms in the coding region of proCPU (Thr147A1a and Thr325I1e) have similar activation kinetics and the activated enzymes have similar carboxypeptidase B-like activity toward small synthetic substrates (26, 28).
We first reported in 1988 on the presence of a labile carboxypeptidase activity in fresh human serum that interfered with the assay of CPN (1-3).
Inactivated serum--serum in which neither activatable proCPU nor basic carboxypeptidase activity could be detected--was prepared by incubating human serum for 12 h at 56 [degrees]C.
2]-terminal sequencing reveal the sequence FQSGQV-LAALPRTSRQVQVL, which confirmed its identity as plasma carboxypeptidase B (7).