TaqMan specific primers and probe consisting of [beta]-Actin, Bax, Bcl-2, Hsp70, survivin, caspase
3, 8 and 9 genes were used in the assay as supplied from TaqMan[R] genes expression assay (Applied Biosystem, USA).
When caspase-8 inactivates RIP1 and RIP3 by proteolytic cleavage, a proapoptotic caspase
activation instead of a pronecrotic cascade is triggered [17-19].
Giaccone, "Cell death independent of caspases
: a review," Clinical Cancer Research, vol.
The findings could lead to the development of nonantibiotic drugs designed to fight certain bacterial infections by activating these caspases
. Those most vulnerable to Legionnaires' disease include the elderly, smokers, and people with chronic diseases or compromised immune systems, including patients with cancer and AIDS.
Synthetic small interfering RNA or silencing RNA, another potential reversal inhibitor of active caspases
, has been introduced as a recent therapeutic modality to ameliorate sepsis-induced immune dysfunction.
To further confirm that caspases
were activated in EBV-induced apoptosis, we assessed XIAP expression.
Ellerby said a substrate based screening method was used to identify compounds that reacted with caspases
. Based on those reactions, Jonathan Ellman, from the Yale University Department of Chemistry, converted the compounds to caspase
The formation of active caspases
forms a cascade in which initiator caspases
(8, 9) interact with the downstream effector molecules (3, 7) to facilitate their own activation.
Identification of caspases
and apoptosis in the simple metazoan Hydra.
Efforts to prevent completion of the programmed cell death process by blocking executioner caspases
have also been reported.
activation was detected in the plasma using an enzyme-linked immunosorbent assay for cytokeratin-18 fragments, which are a byproduct of caspase
Studies of experimental allergic encephalomyelitis (EAE) in caspase
11-deficient mice show that caspasemediated death of OLGs is critical to demyelination.